HighTherm™ Reverse Transcriptase is an engineered mutant of MMLV providing increased specificity and exceptionally high cDNA yields even with complex RNA templates such as viral targets. This novel enzyme is fully functional across a wide temperature range of 38°C to 55°C which enables excellent assay flexibility and high temperature cDNA synthesis for complex RNA secondary structures.
HighTherm™ Reverse Transcriptase efficiently synthesizes a complementary DNA strand from ssRNA, ssDNA or RNA:DNA hybrids with significantly reduced Ribonuclease H activity. HighTherm™ RT generates cDNA from 100 bp to > 12 Kb.
1-Step and 2-Step RT-qPCR, end-point RT-PCR, array labelling, cDNA libraries, 3' and 5' RACE
more representative full-length cDNA due to reduced RNase H activity (100 bp to > 12 Kb)
reproducible cDNA synthesis from a wide range of template (1 pg to 5 µg of total RNA template)
retains greater than 90% of enzymatic activity following 50°C incubation for 60 minutes.
HighTherm™ RT and 5x HighTherm™ Buffer