Proteinase K Solution is provided as a 20 mg/mL solution and is of molecular biology grade. It is a broad-spectrum serine protease with very high specific activity. Proteinase K is a recombinant enzyme expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.
It is active under a wide range of reaction conditions, including elevated temperatures and in the presence of SDS. As a result, Proteinase K is widely used for the digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K is free of exonucleases, endonucleases, and ribonucleases.
• Extraction of DNA and RNA from different starting materials
• Purification of target material from contaminating proteins
• Removal of DNases and RNases during nucleic acids isolation
Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris
Easy to use, 20 mg/mL solution form
Molecular Biology Grade
High activity and exceptional purity
Active at high temperatures (up to 56 °C) and denaturing conditions (e.g. in the presence of urea and/or SDS), which makes it ideal for digesting proteins in variety of applications
Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0)
Low residual DNA content (≤ 10 pg/mg)
Proteinase K is shipped on blue ice and should be stored at -20°C .