Proteinase K Solution

Proteinase K Solution is provided as a 20 mg/mL solution and is of molecular biology grade. It is a broad-spectrum serine protease with very high specific activity. Proteinase K is a recombinant enzyme expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.

Grouped product items
Product Name	Catalog No.	Qty
Proteinase K Solution 5 mL (1 x 5 mL) $110.00	AZ-3201
Proteinase K Solution 25 mL (5 x 5 mL) $480.00	AZ-3205
Proteinase K Solution 100 mL (20 x 5 mL) $1,540.00	AZ-3220

Details

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It is active under a wide range of reaction conditions, including elevated temperatures and in the presence of SDS. As a result, Proteinase K is widely used for the digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K is free of exonucleases, endonucleases, and ribonucleases.

Applications

• Extraction of DNA and RNA from different starting materials
• Purification of target material from contaminating proteins
• Removal of DNases and RNases during nucleic acids isolation

Flexibility

Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris
Easy to use, 20 mg/mL solution form
Molecular Biology Grade
High activity and exceptional purity
Active at high temperatures (up to 56 °C) and denaturing conditions (e.g. in the presence of urea and/or SDS), which makes it ideal for digesting proteins in variety of applications
Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0)
Low residual DNA content (≤ 10 pg/mg)

Storage
Proteinase K is shipped on blue ice and should be stored at -20°C .

Manual