Cloning
Successful cloning and subsequent protein expression requires the selection of the most suitable DNA Polymerase or 2x master-mix. The two most common PCR cloning methods are TA cloning and blunt-end cloning, and the choice depends on the downstream strategy, nature of the vector, and the PCR enzyme. While TA cloning typically employs a thermostable Taq DNA Polymerase, TA cloning with Taq does have limitations such as the size of the insert and the inherent error-rate of this DNA Polymerase. Alternatively, blunt-end cloning involves the ligation of an insert into a linearized vector where both DNA fragments lack overhangs. Blunt-end inserts can be produced using high-fidelity DNA Polymerases with 3′→5′ exonuclease or proofreading activity. This proofreading activity improves the sequence accuracy of the amplified products; however, limitations include lower ligation efficiencies. Azura Genomics offers a range of PCR enzymes and 2x master-mix options to suit the strategies and complexities of cloning.
Successful cloning and subsequent protein expression requires the selection of the most suitable DNA Polymerase or 2x master-mix. The two most common PCR cloning methods are TA cloning and blunt-end cloning, and the choice depends on the downstream strategy, nature of the vector, and the PCR enzyme. While TA cloning typically employs a thermostable Taq DNA Polymerase, TA cloning with Taq does have limitations such as the size of the insert and the inherent error-rate of this DNA Polymerase. Alternatively, blunt-end cloning involves the ligation of an insert into a linearized vector where both DNA fragments lack overhangs. Blunt-end inserts can be produced using high-fidelity DNA Polymerases with 3′→5′ exonuclease or proofreading activity. This proofreading activity improves the sequence accuracy of the amplified products; however, limitations include lower ligation efficiencies. Azura Genomics offers a range of PCR enzymes and 2x master-mix options to suit the strategies and complexities of cloning.
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ExtremeTaq™ DNA PolymeraseExtremeTaq™ DNA Polymerase is an enhanced formulation containing Taq Polymerase, proprietary enhancers, hot-start antibodies, and a proof-reading component for trouble-free PCR reaction assembly and consistent performance. ExtremeTaq Polymerase delivers a unique balance of PCR sensitivity, high fidelity, and value.
Learn MoreStarting at $215.00
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ExtremeTaq™ HiFi MixThe Universal All-in-One PCR Mix for Everyday PCR
ExtremeTaq™ HiFi Mix is an optimized 2x master-mix comprised of an enhanced Taq DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs. This versatile master-mix is ideally suited to all routine end-point PCR applications and challenging DNA targets such as complex GC-rich DNA and low-copy number samples.
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
Learn MoreStarting at $85.00
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ExtremeTaq™ HiFi Red MixThe Universal All-in-One PCR Mix for Everyday PCR.
ExtremeTaq™ HiFi Red Mix is an optimized 2x master-mix comprised of an enhanced Taq DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and inert red gel-loading dye. This versatile master-mix is ideally suited to all routine end-point PCR applications and challenging DNA targets such as complex GC-rich DNA and low-copy number samples.
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
Learn MoreStarting at $85.00
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TruFi™ II DNA PolymeraseTruFi™ II DNA Polymerase is an ultra-high fidelity proofreading DNA polymerase exhibiting both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity, enabling the correction of mismatches during the amplification process.
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
Learn MoreStarting at $135.00
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TruFi™ II Ultra 2x PCR MixTruFi™ II Ultra 2x PCR Mix is an optimized 2x master-mix comprised of TruFi™ II DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs.
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
Learn MoreStarting at $135.00
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TruFi™ II Ultra 2x PCR Red MixTruFi™ II Ultra 2x PCR Red Mix is an optimized 2x master-mix comprised of TruFi™ II DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and an inert red gel-loading dye.
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
Learn MoreStarting at $135.00
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TruFi™ DNA PolymeraseTruFi™ DNA Polymerase is a new generation, ultra high-fidelity DNA polymerase developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions and reaction parameters. Learn MoreStarting at $135.00
Gene Expression & qPCR
End-Point PCR
Electrophoresis
Cloning
Genotyping
Lab Equip & Accessories