Cloning
Successful cloning and subsequent protein expression requires the selection of the most suitable DNA Polymerase or 2x master-mix. The two most common PCR cloning methods are TA cloning and blunt-end cloning, and the choice depends on the downstream strategy, nature of the vector, and the PCR enzyme. While TA cloning typically employs a thermostable Taq DNA Polymerase, TA cloning with Taq does have limitations such as the size of the insert and the inherent error-rate of this DNA Polymerase. Alternatively, blunt-end cloning involves the ligation of an insert into a linearized vector where both DNA fragments lack overhangs. Blunt-end inserts can be produced using high-fidelity DNA Polymerases with 3′→5′ exonuclease or proofreading activity. This proofreading activity improves the sequence accuracy of the amplified products; however, limitations include lower ligation efficiencies. Azura Genomics offers a range of PCR enzymes and 2x master-mix options to suit the strategies and complexities of cloning.
Successful cloning and subsequent protein expression requires the selection of the most suitable DNA Polymerase or 2x master-mix. The two most common PCR cloning methods are TA cloning and blunt-end cloning, and the choice depends on the downstream strategy, nature of the vector, and the PCR enzyme. While TA cloning typically employs a thermostable Taq DNA Polymerase, TA cloning with Taq does have limitations such as the size of the insert and the inherent error-rate of this DNA Polymerase. Alternatively, blunt-end cloning involves the ligation of an insert into a linearized vector where both DNA fragments lack overhangs. Blunt-end inserts can be produced using high-fidelity DNA Polymerases with 3′→5′ exonuclease or proofreading activity. This proofreading activity improves the sequence accuracy of the amplified products; however, limitations include lower ligation efficiencies. Azura Genomics offers a range of PCR enzymes and 2x master-mix options to suit the strategies and complexities of cloning.
-
![]()
ExtremeTaq™ DNA PolymeraseExtremeTaq™ DNA Polymerase is an enhanced formulation containing Taq Polymerase, proprietary enhancers, hot-start antibodies, and a proof-reading component for trouble-free PCR reaction assembly and consistent performance. ExtremeTaq Polymerase delivers a unique balance of PCR sensitivity, high fidelity, and value.
Learn MoreStarting at $215.00
-
![]()
ExtremeTaq™ HiFi MixThe Universal All-in-One PCR Mix for Everyday PCR
ExtremeTaq™ HiFi Mix is an optimized 2x master-mix comprised of an enhanced Taq DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs. This versatile master-mix is ideally suited to all routine end-point PCR applications and challenging DNA targets such as complex GC-rich DNA and low-copy number samples.
Learn MoreStarting at $85.00
-
![]()
ExtremeTaq™ HiFi Red MixThe Universal All-in-One PCR Mix for Everyday PCR.
ExtremeTaq™ HiFi Red Mix is an optimized 2x master-mix comprised of an enhanced Taq DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and inert red gel-loading dye. This versatile master-mix is ideally suited to all routine end-point PCR applications and challenging DNA targets such as complex GC-rich DNA and low-copy number samples.
Learn MoreStarting at $85.00
-
![]()
TruFi™ II DNA PolymeraseTruFi™ II DNA Polymerase is an ultra-high fidelity proofreading DNA polymerase exhibiting both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity, enabling the correction of mismatches during the amplification process. Learn MoreStarting at $101.25
-
![]()
TruFi™ II Ultra 2x PCR MixTruFi™ II Ultra 2x PCR Mix is an optimized 2x master-mix comprised of TruFi™ II DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs. Learn MoreStarting at $135.00
-
![]()
TruFi™ II Ultra 2x PCR Red MixTruFi™ II Ultra 2x PCR Red Mix is an optimized 2x master-mix comprised of TruFi™ II DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and an inert red gel-loading dye. Learn MoreStarting at $135.00
-
![]()
TruFi™ DNA PolymeraseTruFi™ DNA Polymerase is a new generation, ultra high-fidelity DNA polymerase developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions and reaction parameters. Learn MoreStarting at $119.00
Gene Expression & qPCR
End-Point PCR
Electrophoresis
Cloning
Genotyping
Lab Equip & Accessories