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Cloning

Cloning

Successful cloning and subsequent protein expression requires highly efficient DNA transformation. The process of altering a cell so that it acquires and expresses genetic material from a surrounding environment is transformation. This process is commonly used to introduce recombinant plasmid DNA into bacterial strains which can be made competent by artificial means. Chemically competent cells are calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane. Cells are subsequently heat-shocked which opens the pores in the cell membrane so plasmid DNA from the surrounding environment can enter the cell. Electrocompetent cells are prepared for transformation using electroporation for a few milliseconds. This method uses an electrical pulse to create pores in the membrane so that the material can enter the cell from the surrounding environment.

  1. Azura ExtremeTaq

    Azura ExtremeTaq

    Azura ExtremeTaq DNA Polymerase is a high-performance DNA polymerase complex comprised of Azura HS Taq and a proof-reading polymerase in a unique balance which is ideally suited to problematic GC- and AT-rich genomic DNA amplification.


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    Starting at: $145.00

  2. Azura ExtremeTaq 2x Mix

    Azura ExtremeTaq 2x Mix

    Azura ExtremeTaq DNA Polymerase is a high-performance DNA polymerase complex comprised of Azura HS Taq and a proof-reading polymerase in a unique balance which is ideally suited to problematic GC- and AT-rich genomic DNA amplification.


    Learn More

    Starting at: $190.00

  3. TruFi™ DNA Polymerase

    TruFi™ DNA Polymerase

    TruFi™ DNA Polymerase is a new generation, ultra high-fidelity DNA polymerase developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions and reaction parameters.

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    Starting at: $115.00

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