Azura Agarose LE, LM, HR and 3:1
Azura Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications.
Azura Agarose LE
Refined using an advanced process that excludes the use of organic solvents, Agarose LE yields a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kb).The low EEO (≤ 0.13-m) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counter-flow) that can result from the presence of excessive sulfate-rich negative ions. It is easily soluble, free of nucleases, and easy to use. Azura Agarose LE is highly transparent and exhibits exceptionally low absorption of chemical staining agents.
- Nucleic acid electrophoresis (analytical and preparative)
- Protein electrophoresis (including radial immunodiffusion)
- Blotting protocols
Azura Agarose LE Exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added. The low EEO (≤ 0.13-m) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times.
- RNase, DNase and Protease free
- Highly transparent; Ideal for molecular biology applications
Gel strength 8 ≥1200g/cm ²
Gel temperature 36 °C ±1.5 °C (1.5%)
Melt temperature 88 °C ±1.5 °C (1.5%)
EE0 (-mr) 0.13
Moisture content ≤ 7%
Sulfate ≤ 0.2%
Storage conditions Room temperature
Azura Agarose LM, HR and 3:1
- Low Melt (LM): Preparative gels, in gel processing
- Hi-Res (HR): AFLPs STRs, PCR fragments of 20 bp -800 bp
- 3:1 Blend (3:1): AFLPs, STRs, PCR fragments of 50 bp to 1 kb
| Type | LM (Low Melt) | HR (Hi-Res) | 3:1 (Blend) |
| Gel strength | >200 g/cm² (1%) | >750 g/cm² (1.5%) | >650 g/cm² (1.5%) |
| Gel temperature | 26° to 30° C (1.5%) | ≤ 33° C (1.5%) | ≤ 36° C (1.5%) |
| Melt temperature | ≤ 65° C (1.5%) | ≤ 70° C (1.5%) | ≤ 80° C (1.5%) |
| EE0 (-mr) | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% |
| Moisture content | ≤ 10% | ≤ 10% | ≤ 10% |
| Sulfate | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% |
| Resolution | 200bp to 25kbp | 20bp to 800bp | 50bp to 1kbp |
| Storage conditions | Room temperature | Room temperature | Room temperature |
| Applications | Prep Gels, in gel processing | AFLPs, STRs, PCR Small fragments | PCR Blotting |
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