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AzuraQuant™ cDNA Synthesis Kit

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AzuraQuant™ cDNA Synthesis Kit

Quick Overview

The AzuraQuant™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends.

The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm™ reverse transcriptase blended with RNase inhibitor.

In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.

Product Name	Catalog No.	Price
AzuraQuant cDNA Synthesis Kit 25 Reactions	AZ-1995	$89.00
AzuraQuant cDNA Synthesis Kit 100 Reactions	AZ-1996	$299.00
AzuraQuant cDNA Synthesis Kit 1250 Reactions	AZ-1995-50	$2,995.00

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The kit was developed to provide consistent linearity across a wide range of input RNA, revealing accurate relative cDNA representation, regardless of gene abundance. HighTherm™ reverse transcriptase which provides exceptional thermostability is blended with a potent RNase inhibitor to ensure high integrity of Total RNA starting material.

The AzuraQuant™ cDNA Synthesis Kit is supplied with a high-performance 5x buffer system including an optimized ratio of random hexamer primers and anchored oligo (dT) primers which delivers unbiased, efficient and sensitive cDNA synthesis. The AzuraQuant™ cDNA Synthesis Kit delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility and data accuracy. The AzuraQuant™ cDNA Synthesis Kit provides an ideal chemistry for success with limited starting material, such as tissue biopsies.

Applications

2-step RT-qPCR
2-step RT-PCR

Unbiased sensitive cDNA synthesis
Complete 5' to 3' RNA sequence representation from as little as 4pg Total RNA

Reproducible
HighTherm™ reverse transcriptase coupled with an optimized 5x Buffer system generates consistent, high-yield qPCR-ready cDNA.

Broad dynamic range
Optimized for dilute and low-copy input RNA.

Resources

AzuraQuant cDNA Synthesis Kit - Product Sheet Size: (745.87 KB)

AzuraQuant cDNA Synthesis Kit - Manual Size: (536.43 KB)

Customer Reviews

'The AzuraQuant cDNA Synthesis Kit works great! I used 1/10th the amount of input template RNA that I normally use with BioRad and ABI reagents.'

Brown University, Dept. of Biology, Providence, RI, USA