The AzuraQuant™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends.
The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm™ reverse transcriptase blended with RNase inhibitor.
The kit was developed to provide consistent linearity across a wide range of input RNA, revealing accurate relative cDNA representation, regardless of gene abundance. HighTherm™ reverse transcriptase which provides exceptional thermostability is blended with a potent RNase inhibitor to ensure high integrity of Total RNA starting material.
The AzuraQuant™ cDNA Synthesis Kit is supplied with a high-performance 5x buffer system including an optimized ratio of random hexamer primers and anchored oligo (dT) primers which delivers unbiased, efficient and sensitive cDNA synthesis. The AzuraQuant™ cDNA Synthesis Kit delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility and data accuracy. The AzuraQuant™ cDNA Synthesis Kit provides an ideal chemistry for success with limited starting material, such as tissue biopsies.
Complete 5' to 3' RNA sequence representation from as little as 4 pg Total RNA
HighTherm™ reverse transcriptase coupled with an optimized 5x Buffer system generates consistent, high-yield qPCR-ready cDNA.
Optimized for dilute and low-copy input RNA.