Proteinase K

Proteinase K is provided as a lyophilized powder form and is of molecular biology grade. It is a broad-spectrum serine protease with very high specific activity. Proteinase K is a recombinant enzyme expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.

Grouped product items
Product Name	Catalog No.	Qty
Proteinase K 100 mg (1 x 100 mg) $75.00	AZ-3101
Proteinase K 500 mg (5 x 100 mg) $325.00	AZ-3105
Proteinase K 1000 mg (10 x 100 mg) $515.00	AZ-3110

Details

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It is active under a wide range of reaction conditions, including elevated temperatures and in the presence of SDS. As a result, Proteinase K is widely used for the digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K is free of exonucleases, endonucleases, and ribonucleases.

Applications

Extraction of DNA and RNA from different starting materials
Purification of target material from contaminating proteins
Removal of DNases and RNases during nucleic acids isolation

Flexibility

Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris
Molecular Biology Grade
High activity and exceptional purity
Active at high temperatures (up to 56°C) and denaturing conditions (e.g. in the presence of urea and/or SDS), which makes it ideal for digesting proteins in variety of applications
Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0)
Low residual DNA content (≤ 10 pg/mg).

Storage
Proteinase K is shipped on blue ice and should be stored at -20°C .

Manual