Proteinase K
Proteinase K
Azura PureView 100bp DNA Ladder

Azura PureView™ 100 bp DNA Ladder

Proteinase K Solution

Proteinase K Solution

Proteinase K

Proteinase K is provided as a lyophilized powder form and is of molecular biology grade. It is a broad-spectrum serine protease with very high specific activity. Proteinase K is a recombinant enzyme expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.

Grouped product items
Product Name Catalog No. Qty
Proteinase K 100 mg (1 x 100 mg)
$75.00
AZ-3101
Proteinase K 500 mg (5 x 100 mg)
$325.00
AZ-3105
Proteinase K 1000 mg (10 x 100 mg)
$515.00
AZ-3110

It is active under a wide range of reaction conditions, including elevated temperatures and in the presence of SDS. As a result, Proteinase K is widely used for the digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K is free of exonucleases, endonucleases, and ribonucleases.

Applications

  • Extraction of DNA and RNA from different starting materials
  • Purification of target material from contaminating proteins
  • Removal of DNases and RNases during nucleic acids isolation



Flexibility

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris
  • Molecular Biology Grade
  • High activity and exceptional purity
  • Active at high temperatures (up to 56°C) and denaturing conditions (e.g. in the presence of urea and/or SDS), which makes it ideal for digesting proteins in variety of applications
  • Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0)
  • Low residual DNA content (≤ 10 pg/mg).



Storage
Proteinase K is shipped on blue ice and should be stored at -20°C .



Manual
pdf
(Size: 119.5 KB)
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