Toll Free: 888-333-3895

Cart:

0 item(s)

End-Point PCR Applications

End-Point PCR Applications

Invented in 1983 by Kary Mullis, end-point polymerase chain reaction (PCR) enables researchers to expand a single piece of DNA into millions of identical fragments. This well-established technique makes it possible to detect sequences that might otherwise be undetectable. In recent years, the molecular biologist has asked progressively more challenging questions and PCR applications and complexity have evolved. Azura Genomics has developed end-point PCR systems which deliver best-in-class sensitivity, speed and yield. Azura scientists have identified improvements in enzyme solubility and novel buffer additives which enhance reaction efficiency and processivity.

Clearly, our reagents are different and allow the research community to pursue better performance in PCR applications such as crude sample PCR, multiplex PCR, colony PCR, genotyping, GC-rich PCR, long PCR, high-fidelity PCR and cloning.

  1. Azura 1-Step Ultra RT-PCR Kit

    Azura 1-Step Ultra RT-PCR Kit

    Azura One-Step Ultra RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This new generation 2x Mix consists of a thermostable reverse transcriptase, a potent RNase Inhibitor and Azura HS Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

    The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis


    Learn More

    Starting at: $195.00

  2. Azura 2x HS Taq Mix

    Azura 2x HS Taq Mix

    Azura HS Taq DNA Polymerase is an engineered, antibody-mediated Hot-Start PCR enzyme with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.


    Learn More

    Starting at: $145.00

  3. Azura 2x HS Taq Red Mix

    Azura 2x HS Taq Red Mix

    Azura HS Taq DNA Polymerase is an engineered, antibody-mediated Hot-Start PCR enzyme with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.


    Learn More

    Starting at: $145.00

  4. Azura 2x Taq Mix

    Azura 2x Taq Mix

    Azura Taq DNA Polymerase is engineered with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.

    In addition, the ready-to-use mix is available with an inert red dye which allows samples to be loaded directly onto agarose gels without additional gel loading buffers. Azura 2x Taq Red Mix is sufficiently dense to sink to the bottom of the wells.


    Learn More

    Starting at: $85.00

  5. Azura 2x Taq Red Mix

    Azura 2x Taq Red Mix

    Azura Taq DNA Polymerase is engineered with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.

    In addition, the ready-to-use mix is available with an inert red dye which allows samples to be loaded directly onto agarose gels without additional gel loading buffers. Azura 2x Taq Red Mix is sufficiently dense to sink to the bottom of the wells.


    Learn More

    Starting at: $85.00

  6. Azura ExtremeTaq

    Azura ExtremeTaq

    Azura ExtremeTaq DNA Polymerase is a high-performance DNA polymerase complex comprised of Azura HS Taq and a proof-reading polymerase in a unique balance which is ideally suited to problematic GC- and AT-rich genomic DNA amplification.


    Learn More

    Starting at: $145.00

  7. Azura ExtremeTaq 2x Mix

    Azura ExtremeTaq 2x Mix

    Azura ExtremeTaq DNA Polymerase is a high-performance DNA polymerase complex comprised of Azura HS Taq and a proof-reading polymerase in a unique balance which is ideally suited to problematic GC- and AT-rich genomic DNA amplification.


    Learn More

    Starting at: $190.00

  8. Azura HS Taq DNA Polymerase

    Azura HS Taq DNA Polymerase

    Azura HS Taq DNA Polymerase is an engineered, antibody-mediated Hot-Start PCR enzyme with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.


    Learn More

    Starting at: $195.00

  9. Azura Taq DNA Polymerase

    Azura Taq DNA Polymerase

    Azura Taq DNA Polymerase is engineered with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.

    In addition, the ready-to-use mix is available with an inert red dye which allows samples to be loaded directly onto agarose gels without additional gel loading buffers. Azura 2x Taq Red Mix is sufficiently dense to sink to the bottom of the wells.


    Learn More

    Starting at: $75.00

  10. Azura™ Mouse Genotyping Kit

    Azura™ Mouse Genotyping Kit

    Azura™ Mouse Genotyping Kit provides a rapid, low-cost and highly efficient method for the DNA extraction and amplification of a variety of solid tissue types, and is ideally suited to mouse tail snips and mouse ear punches.


    Learn More

    Starting at: $95.00

  11. ChemStart™ DNA Polymerase

    ChemStart™ DNA Polymerase

    ChemStart™ DNA Polymerase is a chemically modified form of Taq DNA Polymerase which requires a heat-activation step prior to PCR. Upon thermal activation, a polymerase modifier is permanently released providing for highly specific enzyme activation.

    ChemStart™ DNA Polymerase exhibits excellent specificity and robust activity with complex viral targets and provides superior performance when compared with alternative polymerase preparations.


    Learn More

    Starting at: $195.00

  12. PureStrand™ Ultra-Pure 100mM dNTP Set

    PureStrand™ Ultra-Pure 100mM dNTP Set

    Individual Ultra-Pure dNTP Solutions of dATP, dCTP, dGTP and dTTP for use in all standard and sensitive PCR applications.


    Learn More

    Starting at: $129.00

  13. PureStrand™ Ultra-Pure 40mM dNTP Mix

    PureStrand™ Ultra-Pure 40mM dNTP Mix

    Ready-to-Use PureStrand 40mM dNTP Mix contains each nucleotide at a 10mM concentration.


    Learn More

    Starting at: $39.00

  14. TruFi™ DNA Polymerase

    TruFi™ DNA Polymerase

    TruFi™ DNA Polymerase is a new generation, ultra high-fidelity DNA polymerase developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions and reaction parameters.

    Learn More

    Starting at: $115.00

My Cart

You have no items in your shopping cart.