End-Point PCR Applications
Invented in 1983 by Kary Mullis, end-point polymerase chain reaction (PCR) enables researchers to expand a single piece of DNA into millions of identical fragments. This well-established technique makes it possible to detect sequences that might otherwise be undetectable. In recent years, the molecular biologist has asked progressively more challenging questions and PCR applications and complexity have evolved. Azura Genomics has developed end-point PCR systems which deliver best-in-class sensitivity, speed and yield. Azura scientists have identified improvements in enzyme solubility and novel buffer additives which enhance reaction efficiency and processivity.
Clearly, our reagents are different and allow the research community to pursue better performance in PCR applications such as crude sample PCR, multiplex PCR, colony PCR, genotyping, GC-rich PCR, long PCR, high-fidelity PCR and cloning.
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Azura 1-Step Ultra RT-PCR Kit
Azura One-Step Ultra RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This new generation 2x Mix consists of a thermostable reverse transcriptase, a potent RNase Inhibitor and Azura HS Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.
The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis
Learn MoreStarting at: $195.00
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Azura 2x HS Taq Mix
Azura HS Taq DNA Polymerase is an engineered, antibody-mediated Hot-Start PCR enzyme with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.
Learn MoreStarting at: $145.00
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Azura 2x HS Taq Red Mix
Azura HS Taq DNA Polymerase is an engineered, antibody-mediated Hot-Start PCR enzyme with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.
Learn MoreStarting at: $145.00
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Azura 2x Taq Mix
Azura Taq DNA Polymerase is engineered with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.
In addition, the ready-to-use mix is available with an inert red dye which allows samples to be loaded directly onto agarose gels without additional gel loading buffers. Azura 2x Taq Red Mix is sufficiently dense to sink to the bottom of the wells.
Learn MoreStarting at: $85.00
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Azura 2x Taq Red Mix
Azura Taq DNA Polymerase is engineered with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.
In addition, the ready-to-use mix is available with an inert red dye which allows samples to be loaded directly onto agarose gels without additional gel loading buffers. Azura 2x Taq Red Mix is sufficiently dense to sink to the bottom of the wells.
Learn MoreStarting at: $85.00
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Azura HS Taq DNA Polymerase
Azura HS Taq DNA Polymerase is an engineered, antibody-mediated Hot-Start PCR enzyme with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.
Learn MoreStarting at: $195.00
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Azura Taq DNA Polymerase
Azura Taq DNA Polymerase is engineered with additional hydrophilic residues for improved solubility and increased affinity for template DNA, resulting in significantly improved activity, yield, speed and sensitivity when compared with wild-type Taq Polymerase.
In addition, the ready-to-use mix is available with an inert red dye which allows samples to be loaded directly onto agarose gels without additional gel loading buffers. Azura 2x Taq Red Mix is sufficiently dense to sink to the bottom of the wells.
Learn MoreStarting at: $75.00
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Azura™ Mouse Genotyping Kit
Azura™ Mouse Genotyping Kit provides a rapid, low-cost and highly efficient method for the DNA extraction and amplification of a variety of solid tissue types, and is ideally suited to mouse tail snips and mouse ear punches.
Learn MoreStarting at: $95.00
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ExtremeTaq HiFi Mix
ExtremeTaq HiFi Mix is a robust, high-fidelity DNA polymerase complex ideally suited to a wide range of DNA templates including the most challenging DNA targets and inhibitor-rich samples.
Learn MoreStarting at: $99.00
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ExtremeTaq HiFi Red Mix
ExtremeTaq HiFi Mix is a robust, high-fidelity DNA polymerase complex ideally suited to a wide range of DNA templates including the most challenging DNA targets and inhibitor-rich samples.
Learn MoreStarting at: $99.00
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PureStrand™ Ultra-Pure 100mM dNTP Set
Individual Ultra-Pure dNTP Solutions of dATP, dCTP, dGTP and dTTP for use in all standard and sensitive PCR applications.
Learn MoreStarting at: $129.00
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Starting at: $39.00
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TruFi™ DNA Polymerase
TruFi™ DNA Polymerase is a new generation, ultra high-fidelity DNA polymerase developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions and reaction parameters.
Learn MoreStarting at: $115.00