End-Point PCR Applications

Invented in 1983 by Kary Mullis, end-point polymerase chain reaction (PCR) enables researchers to expand a single piece of DNA into millions of identical fragments. This well-established technique makes it possible to detect sequences that might otherwise be undetectable. In recent years, the molecular biologist has asked progressively more challenging questions and PCR applications and complexity have evolved. Azura Genomics provides a portfolio of premium-quality PCR enzymes and 2x master-mixes which deliver best-in-class sensitivity, speed and yield. Our technology includes the use of proprietary buffer compositions, stringent hot-start components, and convenient inert visualization and gel-loading dyes to simplify work-flows.

Azura PCR Selection Guide



End-Point PCR Applications

Invented in 1983 by Kary Mullis, end-point polymerase chain reaction (PCR) enables researchers to expand a single piece of DNA into millions of identical fragments. This well-established technique makes it possible to detect sequences that might otherwise be undetectable. In recent years, the molecular biologist has asked progressively more challenging questions and PCR applications and complexity have evolved. Azura Genomics provides a portfolio of premium-quality PCR enzymes and 2x master-mixes which deliver best-in-class sensitivity, speed and yield. Our technology includes the use of proprietary buffer compositions, stringent hot-start components, and convenient inert visualization and gel-loading dyes to simplify work-flows.

Azura PCR Selection Guide



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  1. ExtremeTaq™ HiFi Red Mix

    The Universal All-in-One PCR Mix for Everyday PCR.

    ExtremeTaq™ HiFi Red Mix is an optimized 2x master-mix comprised of an enhanced Taq DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and inert red gel-loading dye. This versatile master-mix is ideally suited to all routine end-point PCR applications and challenging DNA targets such as complex GC-rich DNA and low-copy number samples.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

    Learn More

    Starting at $85.00

  2. ExtremeTaq™ HiFi Mix

    The Universal All-in-One PCR Mix for Everyday PCR

    ExtremeTaq™ HiFi Mix is an optimized 2x master-mix comprised of an enhanced Taq DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs. This versatile master-mix is ideally suited to all routine end-point PCR applications and challenging DNA targets such as complex GC-rich DNA and low-copy number samples.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

    Learn More

    Starting at $85.00

  3. ExtremeTaq™ DNA Polymerase

    ExtremeTaq™ DNA Polymerase is an enhanced formulation containing Taq Polymerase, proprietary enhancers, hot-start antibodies, and a proof-reading component for trouble-free PCR reaction assembly and consistent performance. ExtremeTaq Polymerase delivers a unique balance of PCR sensitivity, high fidelity, and value.

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    Starting at $215.00

  4. Azura HS Taq DNA Polymerase

    Azura HS Taq DNA Polymerase is an antibody-mediated DNA Polymerase which exhibits increased affinity for template DNA and improved specificity. The recombinant hot-start polymerase and proprietary 5x buffer system deliver enhanced sensitivity, DNA yield and reaction speed with a wide range of templates including complex mammalian genomic DNA.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $195.00

  5. Azura 2x HS Taq Red Mix

    Azura 2x HS Taq Red Mix is a ready-to-use master mix optimized for market-leading specificity with a wide range of DNA templates including difficult GC-rich targets.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $165.00

  6. Azura 2x HS Taq Mix

    Azura 2x HS Taq Mix is a ready-to-use master mix optimized for market-leading specificity with a wide range of DNA templates including difficult GC-rich targets.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

    Learn More

    Starting at $165.00

  7. Azura Taq DNA Polymerase

    Azura Taq DNA Polymerase is a highly efficient DNA Polymerase which exhibits increased affinity for template DNA and improved activity.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $75.00

  8. Azura 2x Taq Red Mix

    Azura 2x Taq Red Mix is a ready-to-use master mix optimized for high-yield PCR amplification with a wide range of DNA templates.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $95.00

  9. Azura 2x Taq Mix

    Azura 2x Taq Mix is a ready-to-use master mix optimized for high-yield PCR amplification with a wide range of DNA templates.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $95.00

  10. PureStrand™ Ultra-Pure 40 mM dNTP Mix

    Ready-to-Use PureStrand 40 mM dNTP Mix contains each nucleotide at a 10 mM concentration.

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    Starting at $45.00

  11. PureStrand™ Ultra-Pure 100 mM dNTP Set

    Individual Ultra-Pure dNTP Solutions of dATP, dCTP, dGTP and dTTP for use in all standard and sensitive PCR applications.

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    Starting at $135.00

  12. TruFi™ II DNA Polymerase

    TruFi™ II DNA Polymerase is an ultra-high fidelity proofreading DNA polymerase exhibiting both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity, enabling the correction of mismatches during the amplification process.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $135.00

  13. TruFi™ II Ultra 2x PCR Red Mix

    TruFi™ II Ultra 2x PCR Red Mix is an optimized 2x master-mix comprised of TruFi™ II DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and an inert red gel-loading dye.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

    Learn More

    Starting at $135.00

  14. TruFi™ II Ultra 2x PCR Mix

    TruFi™ II Ultra 2x PCR Mix is an optimized 2x master-mix comprised of TruFi™ II DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $135.00

  15. Azura 1-Step Ultra RT-PCR Kit

    Azura 1-Step Ultra RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This new generation RT-PCR Kit consists of a thermostable reverse transcriptase, a potent RNase Inhibitor and Azura HS Taq for ultra-sensitive 1-step RT-PCR from as little as 1pg total RNA starting material.

    The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $195.00

  16. TruFi™ DNA Polymerase
    TruFi™ DNA Polymerase is a new generation, ultra high-fidelity DNA polymerase developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions and reaction parameters. Learn More

    Starting at $135.00

  17. Flash-Extract™ PCR Kit

    Flash-Extract™ PCR Kit provides for an ultra-fast, low-cost and highly efficient method of DNA extraction and amplification from a variety of solid tissues, including mouse tails and ear punches, plant leaves, bacteria and saliva.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $99.00

  18. Flash-Extract™ Lysis Solution

    Flash-Extract™ Lysis Solution provides for an ultra-fast, low-cost and highly efficient method of DNA extraction from a variety of solid tissues, including mouse tails and ear punches, plant leaves, bacteria and saliva.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $59.00

  19. Azura™ Mouse Genotyping Kit

    Azura™ Mouse Genotyping Kit provides a rapid, low-cost and highly efficient method for the DNA extraction and amplification of a variety of solid tissue types, and is ideally suited to mouse tail snips and mouse ear punches.

    In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

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    Starting at $105.00

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