End-Point PCR Applications

End-Point PCR Applications

Invented in 1983 by Kary Mullis, end-point polymerase chain reaction (PCR) enables researchers to expand a single piece of DNA into millions of identical fragments. This well-established technique makes it possible to detect sequences that might otherwise be undetectable. In recent years, the molecular biologist has asked progressively more challenging questions and PCR applications and complexity have evolved. Azura Genomics has developed end-point PCR systems which deliver best-in-class sensitivity, speed and yield. Azura scientists have identified improvements in enzyme solubility and novel buffer additives which enhance reaction efficiency and processivity.

Clearly, our reagents are different and allow the research community to pursue better performance in PCR applications such as crude sample PCR, multiplex PCR, colony PCR, genotyping, GC-rich PCR, long PCR, high-fidelity PCR and cloning.

Azura PCR Selection Guide



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  1. TruFi™ DNA Polymerase
    TruFi™ DNA Polymerase is a new generation, ultra high-fidelity DNA polymerase developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions and reaction parameters. Learn More

    Starting at $115.00

  2. Azura™ Mouse Genotyping Kit

    Azura™ Mouse Genotyping Kit provides a rapid, low-cost and highly efficient method for the DNA extraction and amplification of a variety of solid tissue types, and is ideally suited to mouse tail snips and mouse ear punches.

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    Starting at $95.00

  3. PureStrand™ Ultra-Pure 40 mM dNTP Mix

    Ready-to-Use PureStrand 40 mM dNTP Mix contains each nucleotide at a 10 mM concentration.

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    Starting at $39.00

  4. PureStrand™ Ultra-Pure 100 mM dNTP Set

    Individual Ultra-Pure dNTP Solutions of dATP, dCTP, dGTP and dTTP for use in all standard and sensitive PCR applications.

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    Starting at $129.00

  5. ExtremeTaq HiFi Red Mix

    ExtremeTaq HiFi Red Mix is a robust, high-fidelity DNA polymerase complex ideally suited to a wide range of DNA templates including the most challenging DNA targets and inhibitor-rich samples.

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    Starting at $99.00

  6. Azura HS Taq DNA Polymerase

    Azura HS Taq DNA Polymerase is an antibody-mediated DNA Polymerase which exhibits increased affinity for template DNA and improved specificity. The recombinant hot-start polymerase and proprietary 5x buffer system deliver enhanced sensitivity, DNA yield and reaction speed with a wide range of templates including complex mammalian genomic DNA.

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    Starting at $195.00

  7. Azura 1-Step Ultra RT-PCR Kit

    Azura 1-Step Ultra RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This new generation RT-PCR Kit consists of a thermostable reverse transcriptase, a potent RNase Inhibitor and Azura HS Taq for ultra-sensitive 1-step RT-PCR from as little as 1pg total RNA starting material.

    The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis

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    Starting at $195.00

  8. Azura Taq DNA Polymerase

    Azura Taq DNA Polymerase is a highly efficient DNA Polymerase which exhibits increased affinity for template DNA and improved activity.

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    Starting at $75.00

  9. Azura 2x Taq Mix

    Azura 2x Taq Mix is a ready-to-use master mix optimized for high-yield PCR amplification with a wide range of DNA templates.

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    Starting at $85.00

  10. Azura 2x Taq Red Mix

    Azura 2x Taq Red Mix is a ready-to-use master mix optimized for high-yield PCR amplification with a wide range of DNA templates.

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    Starting at $85.00

  11. Azura 2x HS Taq Mix

    Azura 2x HS Taq Mix is a ready-to-use master mix optimized for market-leading specificity with a wide range of DNA templates including difficult GC-rich targets.

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    Starting at $145.00

  12. Azura 2x HS Taq Red Mix

    Azura 2x HS Taq Red Mix is a ready-to-use master mix optimized for market-leading specificity with a wide range of DNA templates including difficult GC-rich targets.

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    Starting at $145.00

  13. ExtremeTaq HiFi Mix

    ExtremeTaq HiFi Mix is a robust, high-fidelity DNA polymerase complex ideally suited to a wide range of DNA templates including the most challenging DNA targets and inhibitor-rich samples.

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    Starting at $99.00

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