AzuraFlex™ cDNA Synthesis Kit
The AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability.
The AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability. The enzyme, which is capable of synthesizing cDNA up to 9 Kb is blended with RNase inhibitor for greater stability and performance with a wide range of RNA sources.
- Includes separate solutions of random hexamer primers and oligo (dT) primers for assay flexibility.
- System generates consistent, high-yield qPCR-ready cDNA fragments up to 9Kb in length.
- Optimized for a wide range of input RNA of 10 pg to 2 µg Total RNA.
- Downstream applications include real-time PCR, standard PCR, and microarrays.
- Optimized 5x RTase Buffer Mix includes optimal levels of dNTPs and MgCl2 for reduced pipetting steps.
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