Gene Expression & qPCR
End-Point PCR
Cloning
Genotyping
Lab Equipment and
Electrophoresis
Special OffersThe AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability. The enzyme, which is capable of synthesizing cDNA up to 9Kb is blended with RNase inhibitor for greater stability and performance with a wide range of RNA sources.
- Includes separate solutions of random hexamer primers and oligo (dT) primers for assay flexibility.
- System generates consistent, high-yield qPCR-ready cDNA fragments up to 9Kb in length.
- Optimized for a wide range of input RNA of 10pg to 2µg Total RNA.
- Downstream applications include real-time PCR, standard PCR, and microarrays.
- Optimized 5x RTase Buffer Mix includes optimal levels of dNTPs and MgCl2 for reduced pipetting steps.
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