Azura 1-Step Ultra RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This new generation RT-PCR Kit consists of a thermostable reverse transcriptase, a potent RNase Inhibitor and Azura HS Taq for ultra-sensitive 1-step RT-PCR from as little as 1pg total RNA starting material.
The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
The advanced and highly optimized buffer chemistry allows for efficient reverse transcriptase and PCR of problematic sequences with significant secondary structure (GC-rich targets).
The Azura 1-Step Ultra RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels.
Optimized chemistry for detection of low-copy number transcripts
Overcomes secondary structure in problematic GC-rich targets
First strand full-length cDNA synthesis and PCR in a single tube from a wide input range of total RNA