Azura 1-Step Ultra RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This new generation RT-PCR Kit consists of a thermostable reverse transcriptase, a potent RNase Inhibitor and Azura HS Taq for ultra-sensitive 1-step RT-PCR from as little as 1pg total RNA starting material.
The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis
The advanced and highly optimized buffer chemistry allows for efficient reverse transcriptase and PCR of problematic sequences with significant secondary structure (GC-rich targets).
The Azura 1-Step Ultra RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels.
Optimized chemistry for detection of low-copy number transcripts
Overcomes secondary structure in problematic GC-rich targets
First strand full-length cDNA synthesis and PCR in a single tube from a wide input range of total RNA