Two-Step RT-qPCR

The AzuraQuant™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends.

The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm™ reverse transcriptase blended with RNase inhibitor.

In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.

The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile. The system contains Vivid-Green™ dye, a novel fluorescent DNA-binding dye which produces minimal PCR inhibition and greater fluorescence upon binding to double stranded DNA than SYBR® Green I.

In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.

The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile. The system contains Vivid-Green™ dye, a novel fluorescent DNA-binding dye which produces minimal PCR inhibition and greater fluorescence upon binding to double stranded DNA than SYBR® Green I.

In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.

The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile. The system contains Vivid-Green™ dye, a novel fluorescent DNA-binding dye which produces minimal PCR inhibition and greater fluorescence upon binding to double stranded DNA than SYBR® Green I.

In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.

The AzuraQuant™ Probe Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.

The AzuraQuant™ Probe Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.