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Genotyping

Genotyping

Genetic variation is present in many different forms in the genome, including somatic mutations, single nucleotide polymorphisms (SNPs), copy number variations (CNVs) and structural changes. In order to study and better understand the many variations, Azura Genomics has developed an offering of powerful genotyping tools. High resolution Melt (HRM) and dual-probe based analysis are common methods used in SNP genotyping. We have applied recent advances in enzyme preparation in conjunction with a careful consideration of ionic conditions and buffer components to deliver clear allele discrimination for higher confidence genotyping.

Alternatively, the use of transgenic mice has long been a cornerstone for genetic experimentation. Transgenic mice are commonly used in the lab to model human diseases such as diabetes and cancer. The many challenges of purifying high quality, intact DNA from transgenic mice and subsequently amplifying target genes have been addressed with limited success. Azura Genomics has developed a simple, cost-effective direct PCR platform for high-throughput mouse genotyping. Our polymerase chemistry is ideally suited to high-performance with crude samples, and a broad range of amplicon lengths and sequence complexity.

  1. Azura™ Mouse Genotyping Kit

    Azura™ Mouse Genotyping Kit

    Azura™ Mouse Genotyping Kit provides a rapid, low-cost and highly efficient method for the DNA extraction and amplification of a variety of solid tissue types, and is ideally suited to mouse tail snips and mouse ear punches.


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    Starting at: $95.00

  2. AzuraQuant™ HRM Fast Mix

    AzuraQuant™ HRM Fast Mix

    The AzuraQuant™ HRM Fast Mix is a ready-to-use 2x master mix for use in high-throughput, fast high-resolution melt (HRM) for the analysis of mutations, polymorphisms and epigenetic differences in double-stranded DNA.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


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    Starting at: $65.00

  3. PureView 1kb DNA Ladder

    PureView 1 kb DNA Ladder

    • Easy size determination
    • 13 sharp and bright bands from 250 bp - 10,000 bp
    • Ready-to-use format with high-intensity reference bands at 1000 bp and 3000 bp
    • Room-temperature stable for up to 6 months


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    Starting at: $49.00

  4. PureView 100bp DNA Ladder

    PureView 100 bp DNA Ladder

    • Easy size determination
    • 11 sharp and bright bands from 100 bp – 1.5 kb
    • Ready-to-use format with high-intensity reference bands at 1.5 kb and 500 bp
    • Room-temperature stable for up to 6 months


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    Starting at: $49.00

  5. PureView PCR DNA Ladder

    PureView PCR DNA Ladder

    • Easy size determination
    • 6 sharp and bright bands from 100 bp – 2.0 kb
    • Ready-to-use format with high-intensity reference band at 750 bp
    • Room-temperature stable for up to 6 months
    • Suitable for agarose and polyacrylamide gel electrophoresis


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    Starting at: $49.00

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