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Gene Expression & qPCR

Gene Expression & qPCR

Today, real-time PCR is considered the gold standard for accurate, sensitive and fast measurement of gene expression. Real-Time PCR, also referred to as quantitative PCR (qPCR) was developed as a highly sensitive, rapid and efficient method for nucleic acid detection. This ever-popular technique is based on conventional PCR with the following improvements:

  • qPCR combines amplification and detection in a single step.
  • It has an increased limit of detection and therefore can use smaller amounts of starting material than traditional PCR.
  • The DNA produced can be quantified based on fluorescent detection.

Azura Genomics has engineered real-time PCR solutions which deliver unrivalled sensitivity in low-copy assays and robust performance in the presence of common PCR inhibitors. In addition, our scientists have developed a unique buffer chemistry including novel additives for advanced multiplexing capability, delivering the same high efficiency, early Ct and reaction speed in complex multiplex assays as standard single-plex reactions. The proprietary buffer systems and our enzyme modifications allow for exceptional performance with GC-rich and AT-rich sequences and the option of ultra-fast cycling.

AzuraQuant™ Selection Guide

  1. AzuraFlex™ cDNA Synthesis Kit

    AzuraFlex™ cDNA Synthesis Kit

    The AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability.


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    Starting at: $285.00

  2. AzuraQuant™ cDNA Synthesis Kit

    AzuraQuant™ cDNA Synthesis Kit

    The AzuraQuant™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends.

    The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm™ reverse transcriptase blended with RNase inhibitor.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


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    Starting at: $89.00

  3. AzuraQuant™ Green 1-Step qRT-PCR Kit HiRox

    AzuraQuant™ Green 1-Step qRT-PCR Kit HiRox

    The AzuraQuant™ Green One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


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    Starting at: $145.00

  4. AzuraQuant™ Green 1-Step qRT-PCR Kit LoRox

    AzuraQuant™ Green 1-Step qRT-PCR Kit LoRox

    The AzuraQuant™ Green One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


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    Starting at: $145.00

  5. AzuraQuant™ Green Fast qPCR Mix HiRox

    AzuraQuant™ Green Fast qPCR Mix HiRox

    The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer for use in real-time quantitative PCR assays.

    In order to determine instrument compatibility and the most appropriate formulation, please refer to AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $55.00

  6. AzuraQuant™ Green Fast qPCR Mix LoRox

    AzuraQuant™ Green Fast qPCR Mix LoRox

    The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer for use in real-time quantitative PCR assays.

    In order to determine instrument compatibility and the most appropriate formulation, please refer to AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $55.00

  7. AzuraQuant™ Probe 1-Step qRT-PCR Kit HiRox

    AzuraQuant™ Probe 1-Step qRT-PCR Kit HiRox

    The AzuraQuant™ Probe One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $145.00

  8. AzuraQuant™ Probe 1-Step qRT-PCR Kit LoRox

    AzuraQuant™ Probe 1-Step qRT-PCR Kit LoRox

    The AzuraQuant™ Probe One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $145.00

  9. AzuraQuant™ Probe 1-Step qRT-PCR Kit NoRox

    AzuraQuant™ Probe 1-Step qRT-PCR Kit NoRox

    The AzuraQuant™ Probe One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $145.00

  10. AzuraQuant™ Probe Fast qPCR Mix HiRox

    AzuraQuant™ Probe Fast qPCR Mix HiRox

    The AzuraQuant™ Probe Fast qPCR Mix is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate formulation, please refer toAzuraQuant™ Selection Guide.


    Learn More

    Starting at: $65.00

  11. AzuraQuant™ Probe Fast qPCR Mix LoRox

    AzuraQuant™ Probe Fast qPCR Mix LoRox

    The AzuraQuant™ Probe Fast qPCR Mix is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate formulation, please refer toAzuraQuant™ Selection Guide.


    Learn More

    Starting at: $65.00

  12. AzuraQuant™ Probe Fast qPCR Mix NoRox

    AzuraQuant™ Probe Fast qPCR Mix NoRox

    The AzuraQuant™ Probe Fast qPCR Mix is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate formulation, please refer toAzuraQuant™ Selection Guide.


    Learn More

    Starting at: $65.00

  13. AzuraView Green 1-Step qPCR Blue Mix HR

    AzuraView Green 1-Step qPCR Blue Mix HR

    The Ultra-Stable, Best-in-Class SYBR™ Mix

    The AzuraView Green 1-Step qPCR Blue Mix LR is a ready-to-use 2x master mix and companion thermostable enzyme blend for use in highly sensitive real-time SYBR™ RT-qPCR assays directly from RNA templates. The AzuraView™ Green 1-Step qPCR Blue Mix utilizes an advanced 2x buffer chemistry and a 1-Step enzyme blend of Azura™ HS Taq Polymerase, thermostable Reverse Transcriptase and RNase inhibitor.

    In order to determine instrument compatibility and the most appropriate formulation, please refer to AzuraQuant™ Selection Guide.


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    Starting at: $135.00

  14. AzuraView Green 1-Step qPCR Blue Mix LR

    AzuraView Green 1-Step qPCR Blue Mix LR

    The Ultra-Stable, Best-in-Class SYBR™ Mix

    The AzuraView Green 1-Step qPCR Blue Mix LR is a ready-to-use 2x master mix and companion thermostable enzyme blend for use in highly sensitive real-time SYBR™ RT-qPCR assays directly from RNA templates. The AzuraView™ Green 1-Step qPCR Blue Mix utilizes an advanced 2x buffer chemistry and a 1-Step enzyme blend of Azura™ HS Taq Polymerase, thermostable Reverse Transcriptase and RNase inhibitor.

    In order to determine instrument compatibility and the most appropriate formulation, please refer toAzuraQuant™ Selection Guide.


    Learn More

    Starting at: $135.00

  15. AzuraView™ GreenFast qPCR Blue Mix HR

    AzuraView™ GreenFast qPCR Blue Mix HR

    The Ultra-Stable, Best-in-Class SYBR™ Mix

    The AzuraView™ Green Fast qPCR Blue Mix is an ultrastable 2x master-mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer containing a blue tracer dye for use in SYBR™ real-time quantitative PCR assays.

    In order to determine instrument compatibility and the most appropriate formulation, please refer to AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $45.00

  16. AzuraView™ GreenFast qPCR Blue Mix LR

    AzuraView™ GreenFast qPCR Blue Mix LR

    The Ultra-Stable, Best-in-Class SYBR™ Mix

    The AzuraView™ Green Fast qPCR Blue Mix is an ultrastable 2x master-mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer containing a blue tracer dye for use in SYBR™ real-time quantitative PCR assays.

    In order to determine instrument compatibility and the most appropriate formulation, please refer to AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $45.00

  17. HighTherm™ Reverse Transcriptase

    HighTherm™ Reverse Transcriptase

    HighTherm™ Reverse Transcriptase is an engineered mutant of MMLV providing increased specificity and exceptionally high cDNA yields even with complex RNA templates such as viral targets.

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    Starting at: $165.00

  18. MyGo Mini S Real-Time PCR Instrument

    MyGo Mini S Real-Time PCR Instrument

    The MyGo Mini S is the world's most compact real-time PCR instrument, with no moving parts, enabling silent operation and maximum reliability. It provides users with great results over a broad range of applications.

    Contact us to Learn More

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    $7,999.00
  19. MyGo Pro ESR

    MyGo Pro ESR

    The MyGo Pro real-time PCR instrument provides unmatched performance in a convenient format. Novel Full Spectrum Optics deliver 120 optical channels of fluorescence data from every tube in parallel, with no moving parts, for reliable multiplex PCR.

    Contact us to Learn More

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    $12,999.00

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