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Gene Expression & qPCR

Gene Expression & qPCR

Today, real-time PCR is considered the gold standard for accurate, sensitive and fast measurement of gene expression. Real-Time PCR, also referred to as quantitative PCR (qPCR) was developed as a highly sensitive, rapid and efficient method for nucleic acid detection. This ever-popular technique is based on conventional PCR with the following improvements:

  • qPCR combines amplification and detection in a single step.
  • It has an increased limit of detection and therefore can use smaller amounts of starting material than traditional PCR.
  • The DNA produced can be quantified based on fluorescent detection.

Azura Genomics has engineered real-time PCR solutions which deliver unrivalled sensitivity in low-copy assays and robust performance in the presence of common PCR inhibitors. In addition, our scientists have developed a unique buffer chemistry including novel additives for advanced multiplexing capability, delivering the same high efficiency, early Ct and reaction speed in complex multiplex assays as standard single-plex reactions. The proprietary buffer systems and our enzyme modifications allow for exceptional performance with GC-rich and AT-rich sequences and the option of ultra-fast cycling.

AzuraQuant™ Selection Guide

  1. AzuraFlex™ cDNA Synthesis Kit

    AzuraFlex™ cDNA Synthesis Kit

    The AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability.


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    Starting at: $219.00

  2. AzuraQuant™ cDNA Synthesis Kit

    AzuraQuant™ cDNA Synthesis Kit

    The AzuraQuant™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends.

    The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm™ reverse transcriptase blended with RNase inhibitor.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


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    Starting at: $89.00

  3. AzuraQuant™ Green 1-Step qRT-PCR Kit HiRox

    AzuraQuant™ Green 1-Step qRT-PCR Kit HiRox

    The AzuraQuant™ Green One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $145.00

  4. AzuraQuant™ Green 1-Step qRT-PCR Kit LoRox

    AzuraQuant™ Green 1-Step qRT-PCR Kit LoRox

    The AzuraQuant™ Green One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


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    Starting at: $145.00

  5. AzuraQuant™ Green Fast qPCR Mix Fluor

    AzuraQuant™ Green Fast qPCR Mix Fluor

    The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile. The system contains Vivid-Green™ dye, a novel fluorescent DNA-binding dye which produces minimal PCR inhibition and greater fluorescence upon binding to double stranded DNA than SYBR® Green I.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


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    Starting at: $55.00

  6. AzuraQuant™ Green Fast qPCR Mix HiRox

    AzuraQuant™ Green Fast qPCR Mix HiRox

    The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile. The system contains Vivid-Green™ dye, a novel fluorescent DNA-binding dye which produces minimal PCR inhibition and greater fluorescence upon binding to double stranded DNA than SYBR® Green I.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $55.00

  7. AzuraQuant™ Green Fast qPCR Mix LoRox

    AzuraQuant™ Green Fast qPCR Mix LoRox

    The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile. The system contains Vivid-Green™ dye, a novel fluorescent DNA-binding dye which produces minimal PCR inhibition and greater fluorescence upon binding to double stranded DNA than SYBR® Green I.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $55.00

  8. AzuraQuant™ Probe 1-Step qRT-PCR Kit HiRox

    AzuraQuant™ Probe 1-Step qRT-PCR Kit HiRox

    The AzuraQuant™ Probe One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $145.00

  9. AzuraQuant™ Probe 1-Step qRT-PCR Kit LoRox

    AzuraQuant™ Probe 1-Step qRT-PCR Kit LoRox

    The AzuraQuant™ Probe One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $145.00

  10. AzuraQuant™ Probe 1-Step qRT-PCR Kit NoRox

    AzuraQuant™ Probe 1-Step qRT-PCR Kit NoRox

    The AzuraQuant™ Probe One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $145.00

  11. AzuraQuant™ Probe Fast qPCR Mix HiRox

    AzuraQuant™ Probe Fast qPCR Mix HiRox

    The AzuraQuant™ Probe Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $55.00

  12. AzuraQuant™ Probe Fast qPCR Mix LoRox

    AzuraQuant™ Probe Fast qPCR Mix LoRox

    The AzuraQuant™ Probe Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $55.00

  13. AzuraQuant™ Probe Fast qPCR Mix NoRox

    AzuraQuant™ Probe Fast qPCR Mix NoRox

    The AzuraQuant™ Probe Fast qPCR Mix is a ready-to-use 2x master mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.


    Learn More

    Starting at: $55.00

  14. AzuraView™ GreenFast qPCR Blue Mix HR

    AzuraView™ GreenFast qPCR Blue Mix HR

    The AzuraView™ GreenFast qPCR Blue Mix HR is a ready-to-use, ultra-stable 2x master mix for use in real-time quantitative PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile.


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    Starting at: $65.00

  15. AzuraView™ GreenFast qPCR Blue Mix LR

    AzuraView™ GreenFast qPCR Blue Mix LR

    The AzuraView™ GreenFast qPCR Blue Mix LR is a ready-to-use, ultra-stable 2x master mix for use in real-time quantitative PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile.


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    Starting at: $65.00

  16. HighTherm™ Reverse Transcriptase

    HighTherm™ Reverse Transcriptase

    HighTherm™ Reverse Transcriptase is an engineered mutant of MMLV providing increased specificity and exceptionally high cDNA yields even with complex RNA templates such as viral targets.

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    Starting at: $165.00

  17. MyGo Mini Real-Time PCR Instrument

    MyGo Mini Real-Time PCR Instrument

    The MyGo Mini is the world's most compact real-time PCR instrument, with no moving parts, enabling silent operation and maximum reliability. It provides users with great results over a broad range of applications.

    Schedule a Demo in your Lab

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    $8,295.00
  18. MyGo Pro Real-Time PCR Instrument

    MyGo Pro Real-Time PCR Instrument

    The MyGo Pro real-time PCR instrument provides unmatched performance in a convenient format. Novel Full Spectrum Optics deliver 120 optical channels of fluorescence data from every tube in parallel, with no moving parts, for reliable multiplex PCR.

    Schedule a Demo in your Lab

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    $15,495.00

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