Gene Expression & qPCR

Today, real-time PCR is considered the gold standard for accurate, sensitive and fast measurement of gene expression. Real-Time PCR, also referred to as quantitative PCR (qPCR) was developed as a highly sensitive, rapid and efficient method for nucleic acid detection. This ever-popular technique is based on conventional PCR with the following improvements:

  • qPCR combines amplification and detection in a single step.
  • It has an increased limit of detection and therefore can use smaller amounts of starting material than traditional PCR.
  • The DNA produced can be quantified based on fluorescent detection.

Azura Genomics has engineered real-time PCR solutions which deliver unrivalled sensitivity in low-copy assays and robust performance in the presence of common PCR inhibitors. In addition, our scientists have developed a unique buffer chemistry including novel additives for advanced multiplexing capability, delivering the same high efficiency, early Ct and reaction speed in complex multiplex assays as standard single-plex reactions. The proprietary buffer systems and our enzyme modifications allow for exceptional performance with GC-rich and AT-rich sequences and the option of ultra-fast cycling.

AzuraQuant™ Selection Guide



Gene Expression & qPCR

Today, real-time PCR is considered the gold standard for accurate, sensitive and fast measurement of gene expression. Real-Time PCR, also referred to as quantitative PCR (qPCR) was developed as a highly sensitive, rapid and efficient method for nucleic acid detection. This ever-popular technique is based on conventional PCR with the following improvements:

  • qPCR combines amplification and detection in a single step.
  • It has an increased limit of detection and therefore can use smaller amounts of starting material than traditional PCR.
  • The DNA produced can be quantified based on fluorescent detection.

Azura Genomics has engineered real-time PCR solutions which deliver unrivalled sensitivity in low-copy assays and robust performance in the presence of common PCR inhibitors. In addition, our scientists have developed a unique buffer chemistry including novel additives for advanced multiplexing capability, delivering the same high efficiency, early Ct and reaction speed in complex multiplex assays as standard single-plex reactions. The proprietary buffer systems and our enzyme modifications allow for exceptional performance with GC-rich and AT-rich sequences and the option of ultra-fast cycling.

AzuraQuant™ Selection Guide



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  1. AzuraView™ GreenFast qPCR Blue Mix LR

    The Ultra-Sensitive, Best-in-Class Real Time PCR SYBR® Green Master Mix

    AzuraView™ Green Fast qPCR Blue Mix is an ultra-sensitive 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase, and a highly optimized reaction buffer with a blue tracer dye for use in SYBR® real-time quantitative assays.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $65.00

  2. AzuraView™ GreenFast qPCR Blue Mix HR

    The Ultra-Sensitive, Best-in-Class Real Time PCR SYBR® Green Master Mix

    AzuraView™ Green Fast qPCR Blue Mix is an ultra-sensitive 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase, and a highly optimized reaction buffer with a blue tracer dye for use in SYBR® real-time quantitative assays.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $65.00

  3. AzuraView™ Probe Fast qPCR Mix Blue

    AzuraView™ Probe Fast qPCR Mix Blue is an inhibitor-tolerant 2x master mix for use in real-time quantitative PCR assays, and has been formulated for probe-detection technology, including TaqMan® and molecular beacon probes. The 2x master mix includes a blue tracer dye providing an easy to see, easy to pipette format.

    Learn More

    Starting at $65.00

  4. AzuraQuant™ Green Fast qPCR Mix LoRox

    The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer for use in real-time quantitative PCR assays.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $65.00

  5. AzuraQuant™ Green Fast qPCR Mix HiRox

    The AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer for use in real-time quantitative PCR assays.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $65.00

  6. AzuraQuant™ Green 1-Step qPCR Mix LoRox

    The AzuraQuant™ Green 1-Step qPCR Mix LoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR directly from RNA. The 2x master mix contains a non-inhibitory intercalating dye which provides the option of a post-amplification melt profile.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $115.00

  7. AzuraQuant™ Green 1-Step qPCR Mix HiRox

    The AzuraQuant™ Green 1-Step qPCR Mix HiRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR directly from RNA. The 2x master mix contains a non-inhibitory intercalating dye which provides the option of a post-amplification melt profile.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $115.00

  8. AzuraQuant Probe Fast qPCR Mix NoRox

    The AzuraQuant™ Probe Fast qPCR Mix NoRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $65.00

  9. AzuraQuant Probe Fast qPCR Mix LoRox

    The AzuraQuant Probe Fast qPCR Mix LoRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $65.00

  10. AzuraQuant Probe Fast qPCR Mix HiRox

    The AzuraQuant Probe Fast qPCR Mix HiRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $65.00

  11. AzuraQuant Probe 1-Step qPCR Mix NoRox

    The AzuraQuant™ Probe 1-Step qPCR Mix NoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $115.00

  12. AzuraQuant Probe 1-Step qPCR Mix LoRox

    The AzuraQuant™ Probe 1-Step qPCR Mix LoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $115.00

  13. AzuraQuant Probe 1-Step qPCR Mix HiRox

    The AzuraQuant™ Probe 1-Step qPCR Mix HiRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

    In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.

    Learn More

    Starting at $115.00

  14. AzuraQuant™ II cDNA Synthesis Kit

    The Azura-Quant™ II cDNA Synthesis Kit has been developed for fast and sensitive first-strand cDNA Synthesis. The kit was developed to provide unbiased synthesis across a wide range of input RNA, up to 2 µg.

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    Starting at $125.00

  15. HighTherm™ Reverse Transcriptase
    HighTherm™ Reverse Transcriptase is an engineered mutant of MMLV providing increased specificity and exceptionally high cDNA yields even with complex RNA templates such as viral targets. Learn More

    Starting at $225.00

  16. AzuraQuant™ cDNA Synthesis Kit

    The AzuraQuant™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends.

    The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm™ reverse transcriptase blended with RNase inhibitor.

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    Starting at $125.00

  17. AzuraFlex™ cDNA Synthesis Kit

    The AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability.

    Learn More

    Starting at $335.00

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