Gene Expression & qPCR
Today, real-time PCR is considered the gold standard for accurate, sensitive and fast measurement of gene expression. Real-Time PCR, also referred to as quantitative PCR (qPCR) was developed as a highly sensitive, rapid and efficient method for nucleic acid detection. This ever-popular technique is based on conventional PCR with the following improvements:
- qPCR combines amplification and detection in a single step.
- It has an increased limit of detection and therefore can use smaller amounts of starting material than traditional PCR.
- The DNA produced can be quantified based on fluorescent detection.
Azura Genomics has engineered real-time PCR solutions which deliver unrivalled sensitivity in low-copy assays and robust performance in the presence of common PCR inhibitors. In addition, our scientists have developed a unique buffer chemistry including novel additives for advanced multiplexing capability, delivering the same high efficiency, early Ct and reaction speed in complex multiplex assays as standard single-plex reactions. The proprietary buffer systems and our enzyme modifications allow for exceptional performance with GC-rich and AT-rich sequences and the option of ultra-fast cycling.
Today, real-time PCR is considered the gold standard for accurate, sensitive and fast measurement of gene expression. Real-Time PCR, also referred to as quantitative PCR (qPCR) was developed as a highly sensitive, rapid and efficient method for nucleic acid detection. This ever-popular technique is based on conventional PCR with the following improvements:
- qPCR combines amplification and detection in a single step.
- It has an increased limit of detection and therefore can use smaller amounts of starting material than traditional PCR.
- The DNA produced can be quantified based on fluorescent detection.
Azura Genomics has engineered real-time PCR solutions which deliver unrivalled sensitivity in low-copy assays and robust performance in the presence of common PCR inhibitors. In addition, our scientists have developed a unique buffer chemistry including novel additives for advanced multiplexing capability, delivering the same high efficiency, early Ct and reaction speed in complex multiplex assays as standard single-plex reactions. The proprietary buffer systems and our enzyme modifications allow for exceptional performance with GC-rich and AT-rich sequences and the option of ultra-fast cycling.
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HighTherm™ Reverse TranscriptaseHighTherm™ Reverse Transcriptase is an engineered mutant of MMLV providing increased specificity and exceptionally high cDNA yields even with complex RNA templates such as viral targets. Learn MoreStarting at $195.00
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MyGo Pro ESRThe MyGo Pro real-time PCR instrument provides unmatched performance in a convenient format. Novel Full Spectrum Optics deliver 120 optical channels of fluorescence data from every tube in parallel, with no moving parts, for reliable multiplex PCR.
Contact us to Learn More Learn More$16,499.00 -
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MyGo Mini S Real-Time PCR InstrumentThe MyGo Mini S is the world's most compact real-time PCR instrument, with no moving parts, enabling silent operation and maximum reliability. It provides users with great results over a broad range of applications.
Contact us to Learn More Learn More$7,999.00 -
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AzuraQuant™ cDNA Synthesis KitThe AzuraQuant™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends.
The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm™ reverse transcriptase blended with RNase inhibitor.
Learn MoreStarting at $95.00
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AzuraFlex™ cDNA Synthesis KitThe AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability.
Learn MoreStarting at $325.00
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AzuraView™ GreenFast qPCR Blue Mix HRThe Ultra-Stable, Best-in-Class SYBR™ Mix
The AzuraView™ Green Fast qPCR Blue Mix is an ultrastable 2x master-mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer containing a blue tracer dye for use in SYBR™ real-time quantitative PCR assays.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $59.00
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AzuraView™ GreenFast qPCR Blue Mix LRThe Ultra-Stable, Best-in-Class SYBR™ Mix
The AzuraView™ Green Fast qPCR Blue Mix is an ultrastable 2x master-mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer containing a blue tracer dye for use in SYBR™ real-time quantitative PCR assays.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $59.00
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AzuraQuant Probe 1-Step qPCR Mix HiRoxThe AzuraQuant™ Probe 1-Step qPCR Mix HiRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $145.00
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AzuraQuant Probe 1-Step qPCR Mix NoRoxThe AzuraQuant™ Probe 1-Step qPCR Mix NoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $95.00
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AzuraQuant Probe Fast qPCR Mix LoRoxThe AzuraQuant Probe Fast qPCR Mix LoRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
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AzuraQuant Probe Fast qPCR Mix NoRoxThe AzuraQuant™ Probe Fast qPCR Mix NoRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
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AzuraQuant™ Green 1-Step qPCR Mix LoRoxThe AzuraQuant™ Green 1-Step qPCR Mix LoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR directly from RNA. The 2x master mix contains a non-inhibitory intercalating dye which provides the option of a post-amplification melt profile.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $95.00
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AzuraQuant™ Green Fast qPCR Mix HiRoxThe AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer for use in real-time quantitative PCR assays.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $59.00
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AzuraQuant Probe 1-Step qPCR Mix LoRoxThe AzuraQuant™ Probe 1-Step qPCR Mix LoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $95.00
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AzuraQuant™ Green 1-Step qPCR Mix HiRoxThe AzuraQuant™ Green 1-Step qPCR Mix HiRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR directly from RNA. The 2x master mix contains a non-inhibitory intercalating dye which provides the option of a post-amplification melt profile.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $145.00
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MyGo 0.1 mL Low-Profile TubesMyGo Tubes are ideal for premium performance on MyGo Pro and MyGo Mini Real-Time PCR Systems.
Learn MoreStarting at $155.00
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AzuraQuant™ Green Fast qPCR Mix LoRoxThe AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer for use in real-time quantitative PCR assays.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $59.00
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AzuraQuant Probe Fast qPCR Mix HiRoxThe AzuraQuant Probe Fast qPCR Mix HiRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
Gene Expression & qPCR
End-Point PCR
Electrophoresis
Cloning
Genotyping
Lab Equip & Accessories