Gene Expression & qPCR
Today, real-time PCR is considered the gold standard for accurate, sensitive and fast measurement of gene expression. Real-Time PCR, also referred to as quantitative PCR (qPCR) was developed as a highly sensitive, rapid and efficient method for nucleic acid detection. This ever-popular technique is based on conventional PCR with the following improvements:
- qPCR combines amplification and detection in a single step.
- It has an increased limit of detection and therefore can use smaller amounts of starting material than traditional PCR.
- The DNA produced can be quantified based on fluorescent detection.
Azura Genomics has engineered real-time PCR solutions which deliver unrivalled sensitivity in low-copy assays and robust performance in the presence of common PCR inhibitors. In addition, our scientists have developed a unique buffer chemistry including novel additives for advanced multiplexing capability, delivering the same high efficiency, early Ct and reaction speed in complex multiplex assays as standard single-plex reactions. The proprietary buffer systems and our enzyme modifications allow for exceptional performance with GC-rich and AT-rich sequences and the option of ultra-fast cycling.
Today, real-time PCR is considered the gold standard for accurate, sensitive and fast measurement of gene expression. Real-Time PCR, also referred to as quantitative PCR (qPCR) was developed as a highly sensitive, rapid and efficient method for nucleic acid detection. This ever-popular technique is based on conventional PCR with the following improvements:
- qPCR combines amplification and detection in a single step.
- It has an increased limit of detection and therefore can use smaller amounts of starting material than traditional PCR.
- The DNA produced can be quantified based on fluorescent detection.
Azura Genomics has engineered real-time PCR solutions which deliver unrivalled sensitivity in low-copy assays and robust performance in the presence of common PCR inhibitors. In addition, our scientists have developed a unique buffer chemistry including novel additives for advanced multiplexing capability, delivering the same high efficiency, early Ct and reaction speed in complex multiplex assays as standard single-plex reactions. The proprietary buffer systems and our enzyme modifications allow for exceptional performance with GC-rich and AT-rich sequences and the option of ultra-fast cycling.
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AzuraView™ GreenFast qPCR Blue Mix LRThe Ultra-Sensitive, Best-in-Class Real Time PCR SYBR® Green Master Mix
AzuraView™ Green Fast qPCR Blue Mix is an ultra-sensitive 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase, and a highly optimized reaction buffer with a blue tracer dye for use in SYBR® real-time quantitative assays.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
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AzuraView™ GreenFast qPCR Blue Mix HRThe Ultra-Sensitive, Best-in-Class Real Time PCR SYBR® Green Master Mix
AzuraView™ Green Fast qPCR Blue Mix is an ultra-sensitive 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase, and a highly optimized reaction buffer with a blue tracer dye for use in SYBR® real-time quantitative assays.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
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AzuraView™ Probe Fast qPCR Mix BlueAzuraView™ Probe Fast qPCR Mix Blue is an inhibitor-tolerant 2x master mix for use in real-time quantitative PCR assays, and has been formulated for probe-detection technology, including TaqMan® and molecular beacon probes. The 2x master mix includes a blue tracer dye providing an easy to see, easy to pipette format.
Learn MoreStarting at $65.00
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AzuraQuant™ Green Fast qPCR Mix LoRoxThe AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer for use in real-time quantitative PCR assays.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
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AzuraQuant™ Green Fast qPCR Mix HiRoxThe AzuraQuant™ Green Fast qPCR Mix is a ready-to-use 2x master mix comprised of a non-inhibitory intercalating dye, Azura HS Taq DNA Polymerase and a highly optimized reaction buffer for use in real-time quantitative PCR assays.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
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AzuraQuant™ Green 1-Step qPCR Mix LoRoxThe AzuraQuant™ Green 1-Step qPCR Mix LoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR directly from RNA. The 2x master mix contains a non-inhibitory intercalating dye which provides the option of a post-amplification melt profile.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $115.00
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AzuraQuant™ Green 1-Step qPCR Mix HiRoxThe AzuraQuant™ Green 1-Step qPCR Mix HiRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR directly from RNA. The 2x master mix contains a non-inhibitory intercalating dye which provides the option of a post-amplification melt profile.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $115.00
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AzuraQuant Probe Fast qPCR Mix NoRoxThe AzuraQuant™ Probe Fast qPCR Mix NoRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
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AzuraQuant Probe Fast qPCR Mix LoRoxThe AzuraQuant Probe Fast qPCR Mix LoRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
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AzuraQuant Probe Fast qPCR Mix HiRoxThe AzuraQuant Probe Fast qPCR Mix HiRox is a ready-to-use 2x master-mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $65.00
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AzuraQuant Probe 1-Step qPCR Mix NoRoxThe AzuraQuant™ Probe 1-Step qPCR Mix NoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $115.00
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AzuraQuant Probe 1-Step qPCR Mix LoRoxThe AzuraQuant™ Probe 1-Step qPCR Mix LoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $115.00
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AzuraQuant Probe 1-Step qPCR Mix HiRoxThe AzuraQuant™ Probe 1-Step qPCR Mix HiRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
Learn MoreStarting at $115.00
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AzuraQuant™ II cDNA Synthesis KitThe Azura-Quant™ II cDNA Synthesis Kit has been developed for fast and sensitive first-strand cDNA Synthesis. The kit was developed to provide unbiased synthesis across a wide range of input RNA, up to 2 µg.
Learn MoreStarting at $125.00
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HighTherm™ Reverse TranscriptaseHighTherm™ Reverse Transcriptase is an engineered mutant of MMLV providing increased specificity and exceptionally high cDNA yields even with complex RNA templates such as viral targets. Learn MoreStarting at $225.00
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AzuraQuant™ cDNA Synthesis KitThe AzuraQuant™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends.
The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm™ reverse transcriptase blended with RNase inhibitor.
Learn MoreStarting at $125.00
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AzuraFlex™ cDNA Synthesis KitThe AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability.
Learn MoreStarting at $335.00
Gene Expression & qPCR
End-Point PCR
Electrophoresis
Cloning
Genotyping
Lab Equip & Accessories