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The AzuraQuant™ Green 1-Step qPCR Mix HiRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR directly from RNA. The 2x master mix contains a non-inhibitory intercalating dye which provides the option of a post-amplification melt profile.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
The AzuraQuant™ Green 1-Step qPCR Mix HiRox is powered by Azura HS Taq DNA Polymerase, AzuraSprint Reverse Transcriptase, and an optimized buffer chemistry providing robust first-strand cDNA synthesis and real-time PCR in a single tube.
The Mix delivers early quantification cycle values (Ct) and broad range detection for increased sensitivity, speed, and reproducibility. The AzuraQuant™ Green 1-Step qPCR Mix HiRox can be used to quantify a specific target RNA from either total RNA or mRNA while reducing the number of pipetting steps and time to result.
Compatible with standard and fast cycling instruments and a wide range of cycling parameters.
Efficient One-Tube, One-Step RNA quantification, reducing sample manipulations and experimental variation.
Optimized buffer chemistry for maximum enzyme efficiency allowing detection of low-copy targets with earlier quantification values.