TruFi™ DNA Polymerase
TruFi™ DNA Polymerase TruFi™ DNA Polymerase TruFi™ DNA Polymerase
ExtremeTaq HiFi Mix

ExtremeTaq HiFi Mix

TruFi™ DNA Polymerase

TruFi™ DNA Polymerase is a new generation, ultra high-fidelity DNA polymerase developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions and reaction parameters.

Starting at $115.00

Grouped product items
Product Name Catalog No. Qty
TruFi DNA Polymerase 200u (2u/µl)
$115.00
AZ-1702
TruFi DNA Polymerase 1000u (2u/µl)
$435.00
AZ-1710

TruFi™ DNA Polymerase exhibits robust 5' - 3' DNA polymerase activity and 3' - 5' proof-reading exonuclease activity with an error-rate of 4.55 x 10-7. Several point mutations have selectively modified specific amino acid residues in order to improve protein solubility and performance across a wide range of ionic conditions. TruFi™ DNA Polymerase is provided with a companion 5x reaction buffer delivering superior performance with minimal optimization across a broad range of DNA amplicons, regardless of high AT or GC content. In addition, the hydrophilic nature of TruFi™ provides for significant improvements to reaction processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples.



Applications
High-fidelity PCR for cloning, crude sample PCR, mutagenesis, next-generation re-sequencing, protein expression, microarray, and long PCR up to 10 kb.



Ultra High-Fidelity
> 50-fold higher fidelity than wild-type Taq Polymerase



Robust Amplification
Provides greater yields with lower enzyme amounts than other DNA polymerases, even in crude PCR reactions with known inhibitors.



Convenience of Minimal Optimization
Two-component presentation for ease of use and greater reproducibility - TruFi™ DNA Polymerase is provided with a complete 5x reaction buffer inclusive of GC enhancers, optimal levels of dNTP and MgCl2, and a high ionic strength for broad compatibility with amplicon size and complexity.



Components
TruFi™ Polymerase and 5x TruFi™ Buffer

Manual
Product Sheet
SDS
We found other products you might like!