TruFi™ DNA Polymerase
TruFi™ DNA Polymerase TruFi™ DNA Polymerase TruFi™ DNA Polymerase
ExtremeTaq HiFi Mix

ExtremeTaq HiFi Mix

TruFi™ DNA Polymerase

TruFi™ DNA Polymerase is a new generation, ultra high-fidelity DNA polymerase developed specifically to overcome the many disadvantages of conventional proof-reading enzymes such as poor sensitivity, significant inhibition in crude PCR assays, long extension times and the tedious optimization of buffer conditions and reaction parameters.
Grouped product items
Product Name Catalog No. Qty
TruFi DNA Polymerase 200u (2u/µl)
TruFi DNA Polymerase 1000u (2u/µl)

TruFi™ DNA Polymerase exhibits robust 5' - 3' DNA polymerase activity and 3' - 5' proof-reading exonuclease activity with an error-rate of 4.55 x 10-7. Several point mutations have selectively modified specific amino acid residues in order to improve protein solubility and performance across a wide range of ionic conditions. TruFi™ DNA Polymerase is provided with a companion 5x reaction buffer delivering superior performance with minimal optimization across a broad range of DNA amplicons, regardless of high AT or GC content. In addition, the hydrophilic nature of TruFi™ provides for significant improvements to reaction processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples.

High-fidelity PCR for cloning, crude sample PCR, mutagenesis, next-generation re-sequencing, protein expression, microarray, and long PCR up to 10 kb.

Ultra High-Fidelity
> 50-fold higher fidelity than wild-type Taq Polymerase

Robust Amplification
Provides greater yields with lower enzyme amounts than other DNA polymerases, even in crude PCR reactions with known inhibitors.

Convenience of Minimal Optimization
Two-component presentation for ease of use and greater reproducibility - TruFi™ DNA Polymerase is provided with a complete 5x reaction buffer inclusive of GC enhancers, optimal levels of dNTP and MgCl2, and a high ionic strength for broad compatibility with amplicon size and complexity.

TruFi™ Polymerase and 5x TruFi™ Buffer

Product Sheet
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