ExtremeTaq™ HiFi Mix

The formulation contains Taq Polymerase, proprietary enhancers, hot-start antibodies, and a proof-reading component for trouble-free PCR reaction assembly and consistent performance. ExtremeTaq™ HiFi Mix delivers a unique balance of PCR sensitivity, high fidelity, and value. The ready-to-use ExtremeTaq™ HiFi Mix provides excellent performance with plasmid DNA, cDNA and complex mammalian DNA, and exhibits up to 10x higher fidelity than Taq polymerase. The highly efficient buffer and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature, thereby eliminating non-specific amplification.

To enable convenience in most PCR applications and direct-to-gel analysis following amplification, we also offer ExtremeTaq™ HiFi Red Mix, a 2x master-mix version which includes an inert red gel-loading dye for direct-to-gel analysis. The red gel loading and tracking dye simplifies downstream gel analysis by allowing samples to be loaded directly in agarose gel wells. The red dye migrates at the rate of 600 bp and 350 bp DNA fragments in 1% and 2% TAE agarose gels, respectively.

Applications

• Routine PCR
• Genotyping
• Colony PCR
• PCR of Difficult DNA templates (GC or AT-rich regions)
• Low-copy number PCR
• TA Cloning
• High-fidelity PCR and Long PCR up to 10 kb.

Assay Flexibility and Accuracy
With up to 10x the fidelity of Taq Polymerase, our ready-to-use 2x PCR mix provides robust hot-start PCR in a wide range of applications.

Robust Amplification
Provides greater yields and specificity than other Taq master-mixes, even in low-copy number assays, long PCR up to 10 kb, and in the presence of common PCR inhibitors.

Convenience of Minimal Optimization
ExtremeTaq™ HiFi Mix is designed and optimized for ease-of-use and broad compatibility with DNA templates of various lengths and complexity, without the need for MgCl₂ optimization.

Versatility
High-performance amplification of DNA extracted from human, animal, plant, bacteria, C. elegans, soil and water.