TruFi™ II Ultra 2x PCR Mix is an optimized 2x master-mix comprised of TruFi™ II DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs.
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
TruFi™ II Ultra 2x PCR Mix is ideal for fast cycling, as the polymerase exhibits a rapid elongation rate of 10 seconds per kb. The ready-to-use 2x mix is also highly efficient in long PCR with amplicons greater than 15 kb. With an error rate which is greater than 65x lower than Taq Polymerase, TruFi™ II Ultra 2x PCR Mix is recommended for applications which require extremely high fidelity such as cloning, NGS applications, and mutagenesis.
To enable convenience in most PCR applications and direct-to-gel analysis following amplification, we also offer TruFi™ II Ultra 2x PCR Red Mix.
Ultra-high fidelity PCR for Cloning, Crude Sample PCR, Mutagenesis, NGS Applications, Next-Generation Re-Sequencing, Protein Expression, and Long PCR > 15 kb.
TruFi™ II Ultra 2x PCR Mix exhibits fidelity that is >65x higher than Taq Polymerase and exhibits both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity.
TruFi™ II Ultra 2x PCR Mix provides greater yields with less optimization than other 2x master-mixes, even in amplification of GC-rich targets, DNA of sub-optimal purity, and long templates. TruFi™ II Ultra 2x PCR Mix has a high elongation rate of 10 sec/kb, which is 4x faster than Taq.
Available in 2x ready-to-use colorless and red master-mix format for direct gel-loading and high-throughput.