TruFi™ II Ultra 2x PCR Red Mix is an optimized 2x master-mix comprised of TruFi™ II DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and an inert red gel-loading dye.
In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.
This red gel loading and tracking dye simplifies downstream gel analysis by allowing samples to be loaded directly in agarose gel wells. The red dye front migrates at the rate of 1000 bp - 2000 bp DNA fragments in 0.5% to 1.5% agarose gels. TruFi™ II Ultra 2x PCR Red Mix is ideal for fast cycling, as the polymerase exhibits a rapid elongation rate of 10 seconds per kb. The ready-to-use 2x mix is also highly efficient in long PCR with amplicons greater than 15 kb. With an error rate which is greater than 65x lower than Taq Polymerase, TruFi™ II Ultra 2x PCR Mix is recommended for applications which require extremely high fidelity such as cloning, NGS applications, and mutagenesis.
For applications where a colorless version is preferable, we also offer TruFi™ II Ultra 2x PCR Mix.
Ultra-high fidelity PCR for Cloning, Crude Sample PCR, Mutagenesis, NGS Applications, Next-Generation Re-Sequencing, Protein Expression, and Long PCR > 15 kb.
TruFi™ II Ultra 2x PCR Red Mix exhibits fidelity that is >65x higher than Taq Polymerase and exhibits both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity.
TruFi™ II Ultra 2x PCR Red Mix provides greater yields with less optimization than other 2x master-mixes, even in amplification of GC-rich targets, DNA of sub-optimal purity, and long templates. TruFi™ II Ultra 2x PCR Mix has a high elongation rate of 10 sec/kb, which is 4x faster than Taq.
Available in 2x ready-to-use colorless and red master-mix format for direct gel-loading and high-throughput.