TruFi™ II Ultra 2x PCR Mix
TruFi™ II Ultra 2x PCR Mix TruFi™ II Ultra 2x PCR Mix
TruFi™ II Ultra 2x PCR Red Mix

TruFi™ II Ultra 2x PCR Red Mix

TruFi™ II Ultra 2x PCR Mix

TruFi™ II Ultra 2x PCR Mix is an optimized 2x master-mix comprised of TruFi™ II DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs.

In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

Grouped product items
Product NameCatalog No. Qty
TruFi™ II Ultra 2x PCR Mix 100 Reactions / 2 x 1.25 mL
$135.00
AZ-1920
TruFi™ II Ultra 2x PCR Mix 500 Reactions / 10 x 1.25 mL
$495.00
AZ-1921
TruFi™ II Ultra 2x PCR Mix 1,000 Reactions / 20 x 1.25 mL
$835.00
AZ-1922

TruFi™ II Ultra 2x PCR Mix is ideal for fast cycling, as the polymerase exhibits a rapid elongation rate of 10 seconds per kb. The ready-to-use 2x mix is also highly efficient in long PCR with amplicons greater than 15 kb. With an error rate which is greater than 65x lower than Taq Polymerase, TruFi™ II Ultra 2x PCR Mix is recommended for applications which require extremely high fidelity such as cloning, NGS applications, and mutagenesis.

To enable convenience in most PCR applications and direct-to-gel analysis following amplification, we also offer TruFi™ II Ultra 2x PCR Red Mix.



Applications
Ultra-high fidelity PCR for Cloning, Crude Sample PCR, Mutagenesis, NGS Applications, Next-Generation Re-Sequencing, Protein Expression, and Long PCR > 15 kb.



Ultra High-Fidelity
TruFi™ II Ultra 2x PCR Mix exhibits fidelity that is >65x higher than Taq Polymerase and exhibits both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity.



Robust Amplification
TruFi™ II Ultra 2x PCR Mix provides greater yields with less optimization than other 2x master-mixes, even in amplification of GC-rich targets, DNA of sub-optimal purity, and long templates. TruFi™ II Ultra 2x PCR Mix has a high elongation rate of 10 sec/kb, which is 4x faster than Taq.



Convenience of Minimal Optimization
Available in 2x ready-to-use colorless and red master-mix format for direct gel-loading and high-throughput.



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