TruFi™ II DNA Polymerase

The DNA binding domain of this polymerase ensures robust amplification and more reliable results with a broad range of amplicons (from high AT to high GC). In addition, TruFi™ II DNA Polymerase possesses a rapid elongation rate of 10 seconds per kb, and is highly efficient in long PCR with amplicons greater than 15 kb. With an error rate which is greater than 65x lower than Taq Polymerase, TruFi™ II DNA Polymerase is recommended for applications which require extremely high fidelity such as cloning, NGS applications, and mutagenesis.

To enable convenience in most PCR applications and direct-to-gel analysis following amplification, we also offer TruFi™ II Ultra 2x PCR Red Mix and TruFi II Ultra 2x PCR Mix.

Applications
Ultra-high fidelity PCR for Cloning, Crude Sample PCR, Mutagenesis, NGS Applications, Next-Generation Re-Sequencing, Protein Expression, and Long PCR > 15 kb.

Ultra High-Fidelity
This polymerase has a fidelity that is >65x higher than Taq Polymerase and exhibits both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity.

Robust Amplification
TruFi™ II Polymerase provides greater yields with lower enzyme amounts than other DNA polymerases, even in amplification of GC-rich targets, DNA of sub-optimal purity, and long templates. TruFi™ II Polymerase has a high elongation rate of 10 sec/kb, which is 4x faster than Taq.

Convenience of Minimal Optimization
TruFi™ II DNA Polymerase is supplied with a convenient 5x reaction buffer including an optimal level of MgCl₂. The polymerase is also available in a colorless and red 2x master-mix format for direct gel-loading.