The AzuraQuant™ Probe One-Step qPCR Mix is powered by Azura HS Taq DNA Polymerase, AzuraSprint Reverse Transcriptase, and an optimized buffer chemistry, providing robust first-strand cDNA synthesis and real-time PCR in a single tube. The Mix delivers earlier quantification cycle values (Ct) and broad range detection for increased sensitivity, speed, reliability and reproducibility.
The AzuraQuant™ Probe One-Step qPCR Mix can be used to quantify a specific target RNA from either total RNA or mRNA while reducing the number of pipetting steps and time to result. The AzuraQuant™ Probe One-Step qPCR Mix requires little if any optimization and employs rapid antibody-mediated hot-start activation and a processive enzyme chemistry, providing unmatched performance in muliplexing.
- Multiplex RT-qPCR
- Gene Expression analysis
- Pathogen detection
- Viral Quantitation
- Biomarker discovery
- Gene knock-down validation
- Microarray validation and Detection of extremely low copy targets
Compatible with standard and fast cycling instruments and a wide range of cycling parameters.
Hot-Start chemistry reduces primer-dimer formation and allows room-temperature assembly.
Advanced formulation provides unrivalled sensitivity in singleplex as well as complex multiplex assays.
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