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AzuraQuant™ Green 1-Step qRT-PCR Kit LoRox

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AzuraQuant™ Green 1-Step qRT-PCR Kit LoRox

Quick Overview

The AzuraQuant™ Green One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile.


In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.

Product Name Catalog No. Price Qty
AzuraQuant Green 1-Step qPCR Mix LoRox 100 Reactions AZ-3701
$145.00
AzuraQuant Green 1-Step qPCR Mix LoRox 500 Reactions AZ-3705
$655.00
AzuraQuant Green 1-Step qPCR Mix LoRox 1,000 Reactions AZ-3710
$1,195.00

The system contains Vivid-Green™ dye, a novel fluorescent DNA-binding dye which produces minimal PCR inhibition and greater fluorescence upon binding to double stranded DNA than SYBR® Green I. The AzuraQuant™ Green One-Step qPCR Mix is powered by Azura HS Taq DNA Polymerase and AzuraSprint Reverse Transcriptase, and an optimized buffer chemistry, providing robust first-strand cDNA synthesis and real-time PCR in a single tube.

The Mix delivers earlier quantification cycle values (Ct) and broad range detection for increased sensitivity, speed, reliability and reproducibility. The AzuraQuant™ Green One-Step qPCR Mix can be used to quantify a specific target RNA from either total RNA or mRNA while reducing the number of pipetting steps and time to result.



Applications

  • Gene Expression analysis
  • Pathogen detection
  • Viral Quantitation
  • Biomarker discovery
  • Gene knock-down validation
  • Microarray validation
  • Detection of extremely low copy targets



Flexibility
Compatible with standard and fast cycling instruments and a wide range of cycling parameters.



Accurate Quantification
Efficient One-Tube, One-Step RNA quantification, reducing sample manipulations and experimental variation.



Sensitivity and Speed
Optimized buffer chemistry for maximum enzyme efficiency allowing detection of low-copy targets with earlier quantification values.

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