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AzuraQuant™ Green 1-Step qRT-PCR Kit HiRox

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AzuraQuant™ Green 1-Step qRT-PCR Kit HiRox

Quick Overview

The AzuraQuant™ Green One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays in which intercalating dye-based detection provides the option of a post amplification melt profile.

In order to determine instrument compatibility and the most appropriate ROX™ variant, please click here for AzuraQuant™ Selection Guide.

Product Name	Catalog No.	Price
AzuraQuant Green 1-Step qPCR Mix HiRox 100 Reactions	AZ-3501	$145.00
AzuraQuant Green 1-Step qPCR Mix HiRox 500 Reactions	AZ-3505	$655.00
AzuraQuant Green 1-Step qPCR Mix HiRox 1,000 Reactions	AZ-3510	$1,195.00

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The system contains Vivid-Green™ dye, a novel fluorescent DNA-binding dye which produces minimal PCR inhibition and greater fluorescence upon binding to double stranded DNA than SYBR® Green I. The AzuraQuant™ Green One-Step qPCR Mix is powered by Azura HS Taq DNA Polymerase and AzuraSprint Reverse Transcriptase, and an optimized buffer chemistry, providing robust first-strand cDNA synthesis and real-time PCR in a single tube.

The Mix delivers earlier quantification cycle values (Ct) and broad range detection for increased sensitivity, speed, reliability and reproducibility. The AzuraQuant™ Green One-Step qPCR Mix can be used to quantify a specific target RNA from either total RNA or mRNA while reducing the number of pipetting steps and time to result.

Applications

Gene Expression analysis
Pathogen detection
Viral Quantitation
Biomarker discovery
Gene knock-down validation
Microarray validation
Detection of extremely low copy targets

Flexibility
Compatible with standard and fast cycling instruments and a wide range of cycling parameters.

Accurate Quantification
Efficient One-Tube, One-Step RNA quantification, reducing sample manipulations and experimental variation.

Sensitivity and Speed
Optimized buffer chemistry for maximum enzyme efficiency allowing detection of low-copy targets with earlier quantification values.