The system contains Vivid-Green™ dye, a novel fluorescent DNA-binding dye which produces minimal PCR inhibition and greater fluorescence upon binding to double stranded DNA than SYBR® Green I. The AzuraQuant™ Green One-Step qPCR Mix is powered by Azura HS Taq DNA Polymerase and AzuraSprint Reverse Transcriptase, and an optimized buffer chemistry, providing robust first-strand cDNA synthesis and real-time PCR in a single tube.
The Mix delivers earlier quantification cycle values (Ct) and broad range detection for increased sensitivity, speed, reliability and reproducibility. The AzuraQuant™ Green One-Step qPCR Mix can be used to quantify a specific target RNA from either total RNA or mRNA while reducing the number of pipetting steps and time to result.
- Gene Expression analysis
- Pathogen detection
- Viral Quantitation
- Biomarker discovery
- Gene knock-down validation
- Microarray validation
- Detection of extremely low copy targets
Compatible with standard and fast cycling instruments and a wide range of cycling parameters.
Efficient One-Tube, One-Step RNA quantification, reducing sample manipulations and experimental variation.
Optimized buffer chemistry for maximum enzyme efficiency allowing detection of low-copy targets with earlier quantification values.
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AzuraQuant™ Green Fast qPCR Mix HiRox
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Azura HS Taq DNA Polymerase
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