AzuraProof™ HiFi PCR Mix

AzuraProof™ HiFi PCR Mix is an optimized 2x hot start master mix comprised of AzuraProof™ DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs.  

In order to confirm the ideal reagent for your application, please click here for our PCR Selection Guide.

SKU: AZ-1920
Pack Size: 100 Reactions (2 x 1.25 mL)
Regular price $165.00
Regular price Sale price $165.00
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AzuraProof™ HiFi PCR Mix is ideal for fast cycling, as the polymerase exhibits a rapid elongation rate of 10 seconds per kb. The ready-to-use 2x mix is also highly efficient in long PCR with amplicons greater than 15 kb. With an error rate which is greater than 65x lower than Taq Polymerase, AzuraProof™ HiFi PCR Mix is recommended for applications which require extremely high fidelity such as cloning, NGS applications, and mutagenesis.

To enable convenience in most PCR applications and direct-to-gel analysis following amplification, we also offer AzuraProof™ HiFi PCR Red Mix.

Applications
Ultra-high fidelity PCR for Cloning, Crude Sample PCR, Mutagenesis, NGS Applications, Next-Generation Re-Sequencing, Protein Expression, and Long PCR > 15 kb.

Ultra High-Fidelity
AzuraProof™ HiFi PCR Mix exhibits fidelity that is >65x higher than Taq Polymerase and exhibits both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity.

Robust Amplification
AzuraProof™ HiFi PCR Mix provides greater yields with less optimization than other 2x master-mixes, even in amplification of GC-rich targets, DNA of sub-optimal purity, and long templates. AzuraProof™ HiFi PCR Mix has a high elongation rate of 10 sec/kb, which is 4x faster than Taq.

Convenience of Minimal Optimization
Available in 2x ready-to-use colorless and red master-mix format for direct gel-loading and high-throughput.