AzuraProof™ HiFi PCR Mix

AzuraProof™ HiFi PCR Mix is ideal for fast cycling, as the polymerase exhibits a rapid elongation rate of 10 seconds per kb. The ready-to-use 2x mix is also highly efficient in long PCR with amplicons greater than 15 kb. With an error rate which is greater than 65x lower than Taq Polymerase, AzuraProof™ HiFi PCR Mix is recommended for applications which require extremely high fidelity such as cloning, NGS applications, and mutagenesis.

To enable convenience in most PCR applications and direct-to-gel analysis following amplification, we also offer AzuraProof™ HiFi PCR Red Mix.

Applications
Ultra-high fidelity PCR for Cloning, Crude Sample PCR, Mutagenesis, NGS Applications, Next-Generation Re-Sequencing, Protein Expression, and Long PCR > 15 kb.

Ultra High-Fidelity
AzuraProof™ HiFi PCR Mix exhibits fidelity that is >65x higher than Taq Polymerase and exhibits both 5'→ 3' DNA polymerase activity and 3'→ 5' proofreading exonuclease activity.

Robust Amplification
AzuraProof™ HiFi PCR Mix provides greater yields with less optimization than other 2x master-mixes, even in amplification of GC-rich targets, DNA of sub-optimal purity, and long templates. AzuraProof™ HiFi PCR Mix has a high elongation rate of 10 sec/kb, which is 4x faster than Taq.

Convenience of Minimal Optimization
Available in 2x ready-to-use colorless and red master-mix format for direct gel-loading and high-throughput.