The PureView Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins.
The ladder is supplied in gel loading buffer and is ready to use.
Do NOT heat, dilute, or add reducing agents before loading.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Under suggested conditions, PureView Prestained Protein Ladder resolves 13 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting transfer to a nitrocellulose membrane.
4°C for 3 months
-20°C for 24 months