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Azura ExtremeTaq 2x Mix

Azura ExtremeTaq 2x Mix

Quick Overview

Azura ExtremeTaq DNA Polymerase is a high-performance DNA polymerase complex comprised of Azura HS Taq and a proof-reading polymerase in a unique balance which is ideally suited to problematic GC- and AT-rich genomic DNA amplification.

Product Name Catalog No. Price Qty
ExtremeTaq 2x Mix 200 x 50µl Reactions AZ-1820
$190.00
ExtremeTaq 2x Mix 1000 x 50µl Reactions AZ-1850
$815.00

Azura ExtremeTaq generates extremely high DNA yields and 3'- A overhangs which are ideal for direct integration into TA cloning vectors. In addition, the enzyme delivers exceptional PCR sensitivity in low copy assays with 4-fold higher fidelity than wild-type Taq polymerase. The DNA Polymerase is provided in a simple two-component format for ease of use, minimal handling and greater reproducibility.

Azura ExtremeTaq DNA Polymerase is supplied with a complete 5x reaction buffer inclusive of GC enhancers, optimal levels of dNTP and MgCl2, and a high ionic strength for broad compatibility with amplicon size and complexity. Azura™ ExtremeTaq is also available as a ready-to-use 2x mix formulation containing all of the components including proprietary enhancers for trouble-free PCR reaction assembly while delivering the same unique balance of PCR sensitivity, high fidelity and versatility. The pre-optimized Azura ExtremeTaq 2x Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility.



Applications

  • PCR of difficult DNA templates (high GC and AT content)
  • TA Cloning
  • Low-copy PCR
  • High-fidelity PCR (library amplification)
  • Long PCR up to 10kb



Assay Flexibility
Optimized PCR blend provides robust Hot-Start PCR in a wide range of applications with 4-fold higher fidelity than wild-type Taq Polymerase.



Robust Amplification
Provides greater yields with lower enzyme amounts than other DNA polymerases, even in low-copy PCR reactions and in the presence of common PCR inhibitors.



Convenience of Minimal Optimization
ExtremeTaq DNA Polymerase is provided with a complete 5x reaction buffer inclusive of GC enhancers, optimal levels of dNTP and MgCl2, and a high ionic strength for broad compatibility with amplicon size and complexity. The enzyme blend is also available in a single-tube 2x format for convenience and reproducibility.

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