The AzuraQuant™ Probe 1-Step qPCR Mix HiRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.
In order to confirm instrument compatibility and the most suitable reagent, please click here for qPCR Reagent Compatibility Tool.
The AzuraQuant Probe 1-Step qPCR Mix HiRox is powered by Azura HS Taq DNA Polymerase, AzuraSprint Reverse Transcriptase, and an optimized buffer chemistry, providing robust first-strand cDNA synthesis and real-time PCR in a single tube. The Mix delivers earlier quantification cycle values (Ct) and broad range detection for increased sensitivity, speed, reliability and reproducibility.
The AzuraQuant Probe 1-Step qPCR Mix HiRox can be used to quantify a specific target RNA from either total RNA or mRNA while reducing the number of pipetting steps and time to result. The AzuraQuant Probe 1-Step qPCR Mix HiRox requires little if any optimization and employs rapid antibody-mediated hot-start activation and a processive enzyme chemistry, providing unmatched performance in muliplexing.
Compatible with standard and fast cycling instruments and a wide range of cycling parameters.
Hot-Start chemistry reduces primer-dimer formation and allows room-temperature assembly.
Advanced formulation provides unrivalled sensitivity in singleplex as well as complex multiplex assays.