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ExtremeTaq HiFi Mix

ExtremeTaq HiFi Mix

Quick Overview

ExtremeTaq HiFi Mix is a robust, high-fidelity DNA polymerase complex ideally suited to a wide range of DNA templates including the most challenging DNA targets and inhibitor-rich samples.

Product Name Catalog No. Price Qty
ExtremeTaq HiFi Mix 200 Reactions / 2 x 1.25 mL AZ-1900
ExtremeTaq HiFi Mix 1000 Reactions / 10 x 1.25 mL AZ-1901
ExtremeTaq HiFi Mix 2000 Reactions / 5 x 5 mL AZ-1902

ExtremeTaq HiFi Mix is a hotstart 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase.

The 2x master-mix contains proprietary enhancers, hot-start antibodies and a proof-reading component for trouble-free PCR reaction assembly and performance. ExtremeTaq HiFi Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors. The pre-optimized ExtremeTaq HiFi Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification. ExtremeTaq HiFi Mix is also available with an inert red gel loading and tracking dye allowing samples to be loaded directly in agarose gel wells without separate gel loading buffers. ExtremeTaq HiFi Red Mix contains a red dye which migrates at the rate of 600bp and 350bp DNA in a 1% and 2% TAE agarose gels respectively.


  • PCR of difficult DNA templates (GC- or AT-rich regions) and inhibitor-rich samples
  • TA Cloning
  • low-copy number PCR
  • High-fidelity PCR and Long PCR up to 10kb.

Assay Flexibility and Accuracy
Optimized 2x PCR blend provides robust hot-start PCR in a wide range of applications with 10x higher fidelity than Taq Polymerase.

Robust Amplification
Provides greater yields and specificity than other PCR master-mixes, even in low-copy number assays, long PCR up to 10Kb, and in the presence of common PCR inhibitors.

Convenience of Minimal Optimization
ExtremeTaq HiFi Mix is designed and optimized for ease-of-use and broad compatibility with DNA templates of various lengths and complexity.

High-performance amplification of complex DNA samples extracted from human, animal, plant, bacteria, C. elegans, soil and water.


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